Journal article
Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines
Methods in molecular biology (Clifton, N.J.), Vol.1494, pp.201-213
2017
DOI: 10.1007/978-1-4939-6445-1_14
PMID: 27718195
Abstract
Immune adjuvants, such as ligands for pathogen-associated molecular patterns (PAMPs), have been showing promise in boosting immune responses to tumor associated antigens, and delivering these adjuvants as discrete packages is considered advantageous over delivery in soluble form. Here we describe in detail, methods for independently loading a range of adjuvants into polymer-based biodegradable particles. We also describe the means by which to characterize these particles with respect to adjuvant loading and release kinetics as well as in terms of particle size, shape, and zeta-potential. These adjuvant-loaded particles have the potential to be used in dendritic cell-based uptake experiments performed in vitro or to be used in preclinical cancer vaccine research applications where they can be co-delivered with antigen-loaded particles or some other vaccine component comprising antigenic material.
Details
- Title: Subtitle
- Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines
- Creators
- Cristina Maria de Barros - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USAEmad Ibrahim Wafa - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USAKhanidtha Chitphet - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USAKawther Ahmed - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USASean M Geary - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USAAliasger K Salem - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USA
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.1494, pp.201-213
- DOI
- 10.1007/978-1-4939-6445-1_14
- PMID
- 27718195
- NLM abbreviation
- Methods Mol Biol
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Publisher
- United States
- Grant note
- P30 ES005605 / NIEHS NIH HHS
- Language
- English
- Date published
- 2017
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983985970502771
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