Journal article
Production of antigen-loaded biodegradable nanoparticles and uptake by dendritic cells
Methods in molecular biology (Clifton, N.J.), Vol.1139, pp.453-466
2014
DOI: 10.1007/978-1-4939-0345-0_35
PMID: 24619699
Abstract
Particle-based cancer vaccines prepared from biodegradable polymers are a potentially attractive way of delivering antigen alone, or in combination with adjuvant molecules, to dendritic cells (DC). Non-viral modes of vaccination must ensure the activation of cellular immune responses in addition to humoral responses if an effective antitumor immune response is to be initiated. Here we describe in detail a method for manufacturing poly(D,L-lactide-co-glycolide) (PLGA) particles loaded with purified protein/antigen. We also describe a method for generating bone marrow-derived DC in vitro. These DC can be subsequently used to measure the adjuvant properties of the particle formulations.
Details
- Title: Subtitle
- Production of antigen-loaded biodegradable nanoparticles and uptake by dendritic cells
- Creators
- Vijaya Bharti Joshi - College of Pharmacy, University of Iowa, Iowa City, IA, USASean M GearyAliasger K Salem
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.1139, pp.453-466
- DOI
- 10.1007/978-1-4939-0345-0_35
- PMID
- 24619699
- NLM abbreviation
- Methods Mol Biol
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Publisher
- United States
- Language
- English
- Date published
- 2014
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983985957402771
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