Production of staphylococcal enterotoxin F and pyrogenic exotoxin C by Staphylococcus aureus isolates from toxic shock syndrome-associated sources

P F Bonventre; L Weckbach; J Staneck; P M Schlievert; M Thompson

doi:10.1128/IAI.40.3.1023-1029.1983

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Production of staphylococcal enterotoxin F and pyrogenic exotoxin C by Staphylococcus aureus isolates from toxic shock syndrome-associated sources

Journal article

Open access

Peer reviewed

Production of staphylococcal enterotoxin F and pyrogenic exotoxin C by Staphylococcus aureus isolates from toxic shock syndrome-associated sources

P F Bonventre, L Weckbach, J Staneck, P M Schlievert and M Thompson

Infection and immunity, Vol.40(3), pp.1023-1029

06/1983

DOI: 10.1128/IAI.40.3.1023-1029.1983

PMCID: PMC348153

PMID: 6189784

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Abstract

A total of 136 isolates of Staphylococcus aureus were tested for production of staphylococcal enterotoxin F (SEF) and pyrogenic exotoxin C (PEC), both of which have been identified as reliable indicators of toxic shock syndrome (TSS)-associated strains. SEF and PEC production by isolates from TSS-associated and other sources was tested independently in two laboratories, after which the two sets of data were compared. A 100% concordance between SEF and PEC production was obtained. The TSS toxin candidates were produced by 30 of 136 isolates, and in all instances SEF and PEC were made concurrently by the same strains; in no case was one toxin made and not the other. In the five groups of S. aureus tested, toxins were detected as follows: 23 of 25 (92%) acute TSS isolates, 2 of 48 (4.2%) genital non-TSS isolates, 2 of 16 (12.5%) recovered TSS isolates, 1 of 23 (4.3%) clinical nongenital isolates, and 2 of 24 (8.3%) enterotoxigenic food outbreak isolates. Comparison of purified SEF and purified PEC by immunological and biochemical criteria by immunodiffusion, isoelectric focusing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blot analysis show that the toxins are immunologically identical and strongly suggest that the two nominal TSS toxins are in fact a single protein.

Staphylococcal Food Poisoning - microbiology

Staphylococcus aureus - isolation & purification

Bacterial Toxins - immunology

Exotoxins - immunology

Humans

Epitopes

Genitalia, Female - microbiology

Syndrome

Shock, Septic - microbiology

Bacterial Toxins - biosynthesis

Enterotoxins - biosynthesis

Exotoxins - biosynthesis

Enterotoxins - immunology

Superantigens

Female

Staphylococcus aureus - metabolism

Details

Title: Subtitle: Production of staphylococcal enterotoxin F and pyrogenic exotoxin C by Staphylococcus aureus isolates from toxic shock syndrome-associated sources
Creators: P F Bonventre
L Weckbach
J Staneck
P M Schlievert
M Thompson
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.40(3), pp.1023-1029
Publisher: United States
DOI: 10.1128/IAI.40.3.1023-1029.1983
PMID: 6189784
PMCID: PMC348153
ISSN: 0019-9567
eISSN: 1098-5522
Language: English
Date published: 06/1983
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984002374302771

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