Journal article
Production of staphylococcal pyrogenic exotoxin type C: influence of physical and chemical factors
The Journal of infectious diseases, Vol.147(2), pp.236-242
02/1983
DOI: 10.1093/infdis/147.2.236
PMID: 6827140
Abstract
Physical and chemical factors that may affect the growth of strains of Staphylococcus aureus that are associated with toxic-shock syndrome were examined for their effect on expression of pyrogenic exotoxin type C (PE-C). Significantly more PE-C was made when cultures were incubated at 37 C rather than at 30 C, although bacterial growth was similar at the two temperatures. Furthermore, 32-fold more toxin was made aerobically versus anaerobically for the seven strains tested, whereas only a twofold difference in bacterial growth was seen. Maximal toxin production occurred at pH 7 and pH 8, although the strains grew well in the range of pH 6 to pH 8. Glucose had little effect on growth and toxin production at levels from 0 to 0.3% but suppressed bacterial growth and, more extensively, toxin production at a level of 3%. Significant amounts of PE-C were made in beef-heart medium, brain-heart infusion broth, and Todd-Hewitt broth, but lesser amounts were made in trypticase soy broth.
Details
- Title: Subtitle
- Production of staphylococcal pyrogenic exotoxin type C: influence of physical and chemical factors
- Creators
- P M SchlievertD A Blomster
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.147(2), pp.236-242
- Publisher
- United States
- DOI
- 10.1093/infdis/147.2.236
- PMID
- 6827140
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Grant note
- AI18359 / NIAID NIH HHS
- Language
- English
- Date published
- 02/1983
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001229702771
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