Journal article
Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry
Virology (New York, N.Y.), Vol.375(2), pp.442-451
06/05/2008
DOI: 10.1016/j.virol.2008.01.044
PMCID: PMC2519004
PMID: 18329684
Abstract
Human immunodeficiency virus type 1 (HIV-1) enters dendritic cells (DCs) through endocytosis and viral receptor-mediated fusion. Although endocytosis-mediated HIV-1 entry can generate productive infection in certain cell types, including human monocyte-derived macrophages, productive HIV-1 infection in DCs appears to be dependent on fusion-mediated viral entry. It remains to be defined whether endocytosed HIV-1 in DCs can initiate productive infection. Using HIV-1 infection and cellular fractionation assays to measure productive viral infection and entry, here we show that HIV-1 enters monocyte-derived DCs predominately through endocytosis; however, endocytosed HIV-1 cannot initiate productive HIV-1 infection in DCs. In contrast, productive HIV-1 infection in DCs requires fusion-mediated viral entry. Together, these results provide functional evidence in understanding HIV-1 cis-infection of DCs, suggesting that different pathways of HIV-1 entry into DCs determine the outcome of viral infection.
Details
- Title: Subtitle
- Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry
- Creators
- Alicia M JanasChunsheng DongJian-Hua WangLi Wu
- Resource Type
- Journal article
- Publication Details
- Virology (New York, N.Y.), Vol.375(2), pp.442-451
- DOI
- 10.1016/j.virol.2008.01.044
- PMID
- 18329684
- PMCID
- PMC2519004
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 06/05/2008
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984002391902771
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