Journal article
Profile of a 10-MDP-based universal adhesive system associated with chlorhexidine: Dentin bond strength and in situ zymography performance
Journal of the mechanical behavior of biomedical materials, Vol.110, pp.103925-103925
10/2020
DOI: 10.1016/j.jmbbm.2020.103925
PMID: 32957220
Abstract
The incorporation of functional monomers and proteolytic inhibitors into adhesive systems have shown to be promising strategies to improve the longevity of adhesive restorations. The aim of this study was to evaluate the long-term bonding performance and anti-gelatinolytic effect of a 10-MDP-based universal adhesive system applied in combination with 2% chlorhexidine digluconate (CHX). For that, this study assessed the resin-dentin bond strength and the in situ gelatinolytic activity profile at the adhesive interface at initial and after 6 month of storage. One hundred and two sound human third molars were prepared and randomly divided into 3 groups according to the adhesive strategy: SB (two-step etch-and-rinse adhesive, Adper Single Bond 2, 10-MDP-free control group); SU-ER (Adper Single Bond Universal, 10-MDP containing universal adhesive applied on etch-and-rinse mode); and SU-SE (SU applied on self-etching mode). The groups were subdivided into two according to the dentin pretreatment: W - water or CHX- 2% chlorhexidine digluconate aqueous solution (SB–W; SB-CHX; SU-ER-W; SU-ER-CHX; SU-SE-W; SU-SE-CHX) and subsequently restored according to the manufacturer's instructions. Bond strength (n = 12) was assessed by a microtensile test (μTBS) (500N/0.5 mm/min) after 24h or after 6 months of storage. In situ zymography was performed to evaluate anti-gelatinolytic activity (n = 5). Resin-dentin samples were incubated with fluorescein-conjugated gelatin for 24 h at 37 °C and analyzed by confocal laser scanning microscopy. Fluorescence indicating gelatinolytic activity at hybrid layer zone and adjacencies was quantified using Image J. Data was analyzed by three-way ANOVA and Tukey post-hoc tests (p < 0.05). Results: SU-SE showed the highest bond strength values, while similar results were observed for SU-ER and SB. No statistical significant differences were observed between pretreatment (CHX vs. W) or storage time (initial vs. 6 months of aging). For in situ zymography, fluorescence was detected in all groups and CHX pre-treatment was able to inhibit the gelatinolytic activity in all conditions. The 10-MDP-based universal adhesive system in self-etching mode was the strategy that showed the best bonding performance irrespective of its combination with chlorhexidine. Pre-treatment with CHX did not impair the bond strength when used in combination with 10-MDP and it may promote collagen stability overtime.
Details
- Title: Subtitle
- Profile of a 10-MDP-based universal adhesive system associated with chlorhexidine: Dentin bond strength and in situ zymography performance
- Creators
- Marina Ciccone Giacomini - Universidade de São PauloPolliana Mendes Candia Scaffa - Oregon Health & Science UniversityRafael Simões Gonçalves - Universidade de São PauloGiovanna Speranza Zabeu - Universidade de São PauloCristina de Mattos Pimenta Vidal - Department of Operative Dentistry, College of Dentistry, University of Iowa, 801 Newton Rd, DSB S245, Iowa City, IA, 52242, USAMarcela Rocha de Oliveira Carrilho - College of Dental Medicine Illinois, Midwestern University, Chicago, 555 31st St. Downers Grove, IL, 60515, USAHeitor Marques Honório - Universidade de São PauloLinda Wang - Universidade de São Paulo
- Resource Type
- Journal article
- Publication Details
- Journal of the mechanical behavior of biomedical materials, Vol.110, pp.103925-103925
- DOI
- 10.1016/j.jmbbm.2020.103925
- PMID
- 32957220
- NLM abbreviation
- J Mech Behav Biomed Mater
- ISSN
- 1751-6161
- eISSN
- 1878-0180
- Publisher
- Elsevier Ltd
- Grant note
- DOI: 10.13039/501100002322, name: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil, award: Code 001; DOI: 10.13039/501100003593, name: Conselho Nacional de Desenvolvimento Científico e Tecnológico, award: 163402/2015-9, 408865/2016-4
- Language
- English
- Date published
- 10/2020
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984367641302771
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