Profiles of Exosomal microRNAs in Joint Cells and Candidate microRNAs for Cartilage Regeneration

Venkateswaran Ganesh; Rui He; Henry L Keen; Aliasger K Salem; Edward A Sander; Kyungsup Shin; James A Martin; Dongrim Seol

doi:10.1089/ten.tea.2024.0299

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Profiles of Exosomal microRNAs in Joint Cells and Candidate microRNAs for Cartilage Regeneration

Journal article

Peer reviewed

Profiles of Exosomal microRNAs in Joint Cells and Candidate microRNAs for Cartilage Regeneration

Venkateswaran Ganesh, Rui He, Henry L Keen, Aliasger K Salem, Edward A Sander, Kyungsup Shin, James A Martin and Dongrim Seol

Tissue engineering. Part A, Vol.31(17-18), pp.1132-1143

09/2025

DOI: 10.1089/ten.tea.2024.0299

PMCID: PMC12790756

PMID: 39946223

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https://pmc.ncbi.nlm.nih.gov/articles/PMC12790756/pdf/nihms-2134157.pdfView

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Abstract

The activation of chondrogenic progenitor cells (CPCs) in articular cartilage during a traumatic injury is vital for cartilage regeneration. Although our understanding of the mechanisms underlying CPC chondrogenic activation remains incomplete, there is evidence that exosomal microRNAs (miRNAs or miRs) are involved in tissue healing due to their regulating role of posttranscriptional gene expressions. In this study, we profiled enriched and differential expression of miRNAs in exosomes derived from bovine joint cells (CPCs, chondrocytes, and synoviocytes) via Next Generation Sequencing analysis and validated the potential therapeutic effects of candidate exosomal miRNAs for cartilage regeneration. For CPC-based cartilage regeneration, we tested the impact of administering miR-107, miR-140, and miR-148a on CPCs because we found that these miRNAs were highly and differentially expressed in chondrocytes-derived exosomes (CC-Exo). We found that: (1) miR-140 induced chondrogenic gene expression including SRY-box transcription factor 9, collagen type 2A1, and aggrecan, and (2) miR-107 suppressed catabolic gene expression including matrix metalloproteinase 3, a disintegrin and metalloproteinase with thrombospondin motifs 5, and nitric oxide synthase 2. Our findings indicate that transfection of CPCs with specific chondrogenic miRNAs present in CC-Exo have the potential to promote CPC-based cartilage regeneration and could be an important component of posttraumatic osteoarthritis prevention.

synoviocyte

chondrogenic progenitor cell

chondrocyte

posttraumatic osteoarthritis

exosomal microRNA

articular cartilage regeneration

Details

Title: Subtitle: Profiles of Exosomal microRNAs in Joint Cells and Candidate microRNAs for Cartilage Regeneration
Creators: Venkateswaran Ganesh - University of Iowa
Rui He - University of Iowa
Henry L Keen - University of Iowa
Aliasger K Salem - University of Iowa
Edward A Sander - University of Iowa
Kyungsup Shin - University of Iowa
James A Martin - University of Iowa
Dongrim Seol - University of Iowa
Resource Type: Journal article
Publication Details: Tissue engineering. Part A, Vol.31(17-18), pp.1132-1143
DOI: 10.1089/ten.tea.2024.0299
PMID: 39946223
PMCID: PMC12790756
NLM abbreviation: Tissue Eng Part A
ISSN: 1937-3341
eISSN: 1937-335X
Publisher: MARY ANN LIEBERT, INC
Grant note: Department of Orthopedics and Rehabilitation at the University of Iowa
This work was supported by the Department of Orthopedics and Rehabilitation at the University of Iowa.
Language: English
Electronic publication date: 02/13/2025
Date published: 09/2025
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Orthodontics; Research Administration; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Iowa Institute of Human Genetics
Record Identifier: 9984790978102771

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