Prognostic value of quantifying apoptosis factor expression in favorable histology wilms tumors

Michal A Miller; Bahri Karacay; Norman E Breslow; Sierra Li; M Sue O'Dorisio; Paul E Grundy; Anthony D Sandler

doi:10.1097/01.mph.0000149961.71266.27

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Prognostic value of quantifying apoptosis factor expression in favorable histology wilms tumors

Journal article

Peer reviewed

Prognostic value of quantifying apoptosis factor expression in favorable histology wilms tumors

Michal A Miller, Bahri Karacay, Norman E Breslow, Sierra Li, M Sue O'Dorisio, Paul E Grundy and Anthony D Sandler

Journal of pediatric hematology/oncology, Vol.27(1), pp.11-14

01/2005

DOI: 10.1097/01.mph.0000149961.71266.27

PMID: 15654272

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Abstract

Important in the homeostasis of normal tissues, apoptosis can be altered to favor cell survival within tumors. High expression of survivin, an inhibitor of apoptosis, and absence of caspase 8, a pro-apoptotic enzyme, independently correlate with poor outcomes in several tumor types. Favorable histology Wilms tumor has a remarkably high cure rate; as a result, the focus of therapy is now aimed at reducing treatment-related morbidity. With the goal of safely reducing therapy in select subgroups of patients, the authors investigated whether the levels of apoptotic factors in tumors could predict the risk for recurrence. Tumor apoptotic factor levels were surveyed in a case-control study from the National Wilms Tumor Study 5 (NWTS-5) and measured via quantitative real-time RT-PCR. Survivin and caspase 8 levels were surveyed in 92 primary tumor specimens and SMAC, Bid, and CD95 were surveyed in 24 specimens. All four pro-apoptotic factors studied (caspase 8, SMAC, Bid, and CD95) were analyzed individually and in relation to survivin expression. Although survivin mRNA was present at markedly greater levels than in normal kidney, none of the factors assayed independently or as a ratio was associated with stage of disease or risk for tumor recurrence in this group of tumors.

Gene Expression

Caspase 8

Prognosis

Inhibitor of Apoptosis Proteins

Biomarkers, Tumor - analysis

Microtubule-Associated Proteins - metabolism

BH3 Interacting Domain Death Agonist Protein

RNA, Messenger - analysis

Kidney Neoplasms - metabolism

Reverse Transcriptase Polymerase Chain Reaction

fas Receptor - metabolism

Case-Control Studies

Wilms Tumor - pathology

Carrier Proteins - metabolism

Caspases - metabolism

Mitochondrial Proteins - metabolism

Neoplasm Proteins

Kidney Neoplasms - pathology

Apoptosis - physiology

Wilms Tumor - metabolism

Details

Title: Subtitle: Prognostic value of quantifying apoptosis factor expression in favorable histology wilms tumors
Creators: Michal A Miller - Department of Surgery, University of Iowa, Iowa City, Iowa 52242, USA
Bahri Karacay
Norman E Breslow
Sierra Li
M Sue O'Dorisio
Paul E Grundy
Anthony D Sandler
Resource Type: Journal article
Publication Details: Journal of pediatric hematology/oncology, Vol.27(1), pp.11-14
Publisher: United States
DOI: 10.1097/01.mph.0000149961.71266.27
PMID: 15654272
ISSN: 1077-4114
eISSN: 1536-3678
Grant note: T-32 HLO7344 / NHLBI NIH HHS R01EB002128-04 / NIBIB NIH HHS
Language: English
Date published: 01/2005
Academic Unit: Stead Family Department of Pediatrics; Iowa Neuroscience Institute
Record Identifier: 9984065836602771

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