Journal article
Progress and prospects: techniques for site-directed mutagenesis in animal models
Gene therapy, Vol.16(5), pp.581-588
05/2009
DOI: 10.1038/gt.2009.16
PMCID: PMC2798581
PMID: 19225549
Abstract
In the past 2 years, new gene-targeting approaches using adeno-associated virus and designer zinc-finger nucleases have been successfully applied to the production of genetically modified ferrets, pigs, mice and zebrafish. Gene targeting using these tools has been combined with somatic cell nuclear transfer and germ cell transplantation to generate gene-targeted animal models. These new technical advances, which do not require the generation of embryonic stem cell-derived chimeras, will greatly accelerate the production of non-mouse animal models for biomedical research.
Details
- Title: Subtitle
- Progress and prospects: techniques for site-directed mutagenesis in animal models
- Creators
- Z Yan - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USAX Sun - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USAJ F Engelhardt - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Gene therapy, Vol.16(5), pp.581-588
- DOI
- 10.1038/gt.2009.16
- PMID
- 19225549
- PMCID
- PMC2798581
- NLM abbreviation
- Gene Ther
- ISSN
- 0969-7128
- eISSN
- 1476-5462
- Language
- English
- Date published
- 05/2009
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025455302771
Metrics
20 Record Views