Journal article
Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes
Biotechnology journal, Vol.13(9), pp.e1700591-n/a
09/2018
DOI: 10.1002/biot.201700591
PMID: 29396999
Abstract
The carbon footprint caused by unsustainable development and its environmental and economic impact has become a major concern in the past few decades. Photosynthetic microbes such as microalgae and cyanobacteria are capable of accumulating value-added compounds from carbon dioxide, and have been regarded as environmentally friendly alternatives to reduce the usage of fossil fuels, thereby contributing to reducing the carbon footprint. This light-driven generation of green chemicals and biofuels has triggered the research for metabolic engineering of these photosynthetic microbes. CRISPR-Cas systems are successfully implemented across a wide range of prokaryotic and eukaryotic species for efficient genome editing. However, the inception of this genome editing tool in microalgal and cyanobacterial species took off rather slowly due to various complications. In this review, we elaborate on the established CRISPR-Cas based genome editing in various microalgal and cyanobacterial species. The complications associated with CRISPR-Cas based genome editing in these species are addressed along with possible strategies to overcome these issues. It is anticipated that in the near future this will result in improving and expanding the microalgal and cyanobacterial genome engineering toolbox.
Details
- Title: Subtitle
- Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes
- Creators
- Mihris Ibnu Saleem Naduthodi - Wageningen University & ResearchMaria J. Barbosa - Wageningen University & ResearchJohn van der Oost - Wageningen University & Research
- Resource Type
- Journal article
- Publication Details
- Biotechnology journal, Vol.13(9), pp.e1700591-n/a
- DOI
- 10.1002/biot.201700591
- PMID
- 29396999
- NLM abbreviation
- Biotechnol J
- ISSN
- 1860-6768
- eISSN
- 1860-7314
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- 745754 / project titled "Microalgae As a Green source from Nutritional Ingredients for Food/Feed and Ingredients for Cosmetics by cost effective New Technologies" [MAGNIFICENT] - Bio-based Industries Joint Technology Initiative under European Commission
- Language
- English
- Date published
- 09/2018
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984398022902771
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