Journal article
Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer
Reproductive biology and endocrinology, Vol.1(1), pp.83-83
11/07/2003
DOI: 10.1186/1477-7827-1-83
PMCID: PMC280727
PMID: 14613541
Abstract
Mammalian cloning by nuclear transfer from somatic cells has created new opportunities to generate animal models of genetic diseases in species other than mice. Although genetic mouse models play a critical role in basic and applied research for numerous diseases, often mouse models do not adequately reproduce the human disease phenotype. Cystic fibrosis (CF) is one such disease. Targeted ablation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene in mice does not adequately replicate spontaneous bacterial infections observed in the human CF lung. Hence, several laboratories are pursuing alternative animal models of CF in larger species such as the pig, sheep, rabbits, and ferrets. Our laboratory has focused on developing the ferret as a CF animal model. Over the past few years, we have investigated several experimental parameters required for gene targeting and nuclear transfer (NT) cloning in the ferret using somatic cells. In this review, we will discuss our progress and the hurdles to NT cloning and gene-targeting that accompany efforts to generate animal models of genetic diseases in species such as the ferret.
Details
- Title: Subtitle
- Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer
- Creators
- Ziyi Li - Department of Anatomy & Cell Biology, College of Medicine, University of Iowa, 1-111 BSB, 51 Newton Road, Iowa City, IA 52242, USAJohn F Engelhardt - Department of Anatomy & Cell Biology, College of Medicine, University of Iowa, 1-111 BSB, 51 Newton Road, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Reproductive biology and endocrinology, Vol.1(1), pp.83-83
- Publisher
- BioMed Central; London
- DOI
- 10.1186/1477-7827-1-83
- PMID
- 14613541
- PMCID
- PMC280727
- ISSN
- 1477-7827
- eISSN
- 1477-7827
- Language
- English
- Date published
- 11/07/2003
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025456502771
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