Journal article
Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics
Biomaterials, Vol.27(33), pp.5651-5657
2006
DOI: 10.1016/j.biomaterials.2006.07.027
PMID: 16904740
Abstract
In the present study, the effects of a calcium magnesium silicate bioactive ceramic (akermanite) on proliferation and osteoblastic differentiation of human bone marrow stromal cells (hBMSC) have been investigated and compared with the classical ceramic (
β-tricalcium phosphate,
β-TCP). Akermanite and
β-TCP disks were seeded with hBMSC and kept in growth medium or osteogenic medium for 10 days. Proliferation and osteoblastic differentiation were evaluated on day 1, 4, 7 and 10. The data from the Alamar Blue assay and lactic acid production assay showed that hBMSC proliferated more significantly on akermanite than on
β-TCP. The analysis of osteoblast-related genes, including alkaline phosphatase (ALP), osteopontin (OPN), bone sialoprotein (BSP) and osteocalcin (OC), indicated that akermanite ceramics enhanced the expression of osteoblast-related genes, but type I collagen (COL I) showed no noticeable difference among akermanite and
β-TCP ceramics. Furthermore, this stimulatory effect was observed not only in osteogenic medium, but also in normal growth medium without osteogenic reagents such as
l-ascorbic acid, glycerophosphate and dexamethasone. This result suggests that akermanite can promote osteoblastic differentiation of hBMSC in vitro even without osteogenic reagents, and may be used as a bioactive material for bone regeneration and tissue engineering applications.
Details
- Title: Subtitle
- Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics
- Creators
- Hongli Sun - Orthopaedic Cellular & Molecular Biology Laboratory, Laboratory of Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences & Shanghai JiaoTong University School of Medicine, People's Republic of China.Chengtie Wu - Shanghai Institute of CeramicsKerong Dai - Shanghai Ninth People's HospitalJiang Chang - Shanghai Institute of CeramicsTingting Tang - Shanghai Ninth People's Hospital
- Resource Type
- Journal article
- Publication Details
- Biomaterials, Vol.27(33), pp.5651-5657
- DOI
- 10.1016/j.biomaterials.2006.07.027
- PMID
- 16904740
- NLM abbreviation
- Biomaterials
- ISSN
- 0142-9612
- eISSN
- 1878-5905
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 2006
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery
- Record Identifier
- 9984367580902771
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