Proliferative action of erythropoietin is associated with rapid protein tyrosine phosphorylation in responsive B6SUt.EP cells

Frederick W Quelle; Don M Wojchowski

doi:10.1016/S0021-9258(18)52478-9

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Proliferative action of erythropoietin is associated with rapid protein tyrosine phosphorylation in responsive B6SUt.EP cells

Journal article

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Proliferative action of erythropoietin is associated with rapid protein tyrosine phosphorylation in responsive B6SUt.EP cells

Frederick W Quelle and Don M Wojchowski

The Journal of biological chemistry, Vol.266(1), pp.609-614

01/05/1991

DOI: 10.1016/S0021-9258(18)52478-9

PMID: 1985918

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Abstract

Erythropoietin is a prime regulator of the growth and terminal differentiation of erythroid blood cells. However, little is understood concerning its molecular mechanism of action. Presently it is shown in the responsive, factor-dependent murine cell line B6SUt.EP that erythropoietin induces the tyrosine phosphorylation of six plasma membrane-associated proteins in a time- and concentration-dependent fashion (i.e. phosphoproteins PY153, PY140, PY100, PY93, PY74, and PY54). Among these, PY153 was prominent. For all proteins, maximal levels of phosphorylation were induced within 3-7 min at low factor concentrations (100-500 pM). These findings establish tyrosine kinase activation as a novel candidate pathway of erythropoietin-induced proliferation. In addition, the tyrosine phosphorylation of six proteins with identical Mr, as well as a Mr 104,000 protein, was induced in B6SUt.EP cells by interleukin 3. In contrast, no induced tyrosine phosphorylation was detectable in the erythropoietin-responsive, leukemic erythroid cell line. Rauscher Red 1, yet proteins of Mr 153,000 and 54,000 were shown to be phosphorylated constitutively at relative levels greater than those observed in B6SUt.EP cells. A possible role for these phosphoproteins in hematopoietic cell transformation is considered.

Erythropoietin - pharmacology

Tyrosine

Cell Line

Membrane Proteins - isolation & purification

Interleukin-3 - pharmacology

Cell Division - drug effects

Animals

Phosphoproteins - isolation & purification

Cell Membrane - metabolism

Clone Cells

Membrane Proteins - metabolism

Mice

Erythropoietin - metabolism

Kinetics

Details

Title: Subtitle: Proliferative action of erythropoietin is associated with rapid protein tyrosine phosphorylation in responsive B6SUt.EP cells
Creators: Frederick W Quelle - Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802
Don M Wojchowski
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.266(1), pp.609-614
DOI: 10.1016/S0021-9258(18)52478-9
PMID: 1985918
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: R29 DK40242 / NIDDK NIH HHS
Language: English
Date published: 01/05/1991
Academic Unit: Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040214402771

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