Journal article
Proliferative characteristics of primary and metastatic human solid tumors by DNA flow cytometry
Cytometry (New York, N.Y.), Vol.5(6), pp.629-635
1984
DOI: 10.1002/cyto.990050612
PMID: 6518938
Abstract
The percentage of cells in S-phase (S-index) was calculated from DNA histograms of 453 primary and metastatic human solid tumors (predominantly bladder, breast, colorectal, renal, prostate, ovarian and lung carcinomas, melanomas, and sarcomas). S-indices varied widely among both primary and metastatic tumors (1–48%); there was no significant difference in S-indices between primary and metastatic tumors. The S-indices for aneuploid tumors were signifycantly higher than for diploid tumors. When data for all aneuploid tumors were analyzed collectively, there was no significant relationship between S-index and DNA ploidy index. However, for colorectal and ovarian carcinomas S-indices increased, and for lung carcinomas S-indices decreased with elevation in the degree of DNA ploidy. Lung carcinomas had the highest S-indices. Comparison of flow cytometry (FCM) and cytology data indicated that for most diploid tumors S-indices reflect the proportion of S-phase cells among a mixed population of normal and tumor cells. For most aneuploid tumors, the proportion of tumor cells estimated cytologically was similar to the proportion of aneuploid cells estimated by FCM. For a small proportion of aneuploid tumors a comparison of cytology and FCM data indicated the presence of a predominant diploid tumor stemline and a minor stemline with aneuploid DNA content. There was a wide spread in the values of S-indices within tumor groups defined by degree of differentiation and stage of disease at surgery.
Details
- Title: Subtitle
- Proliferative characteristics of primary and metastatic human solid tumors by DNA flow cytometry
- Creators
- O. S Frankfurt - Roswell Park Cancer InstituteW. R Greco - Roswell Park Cancer InstituteH. K Slocum - Roswell Park Cancer InstituteS. G Arbuck - Roswell Park Cancer InstituteM Gamarra - Roswell Park Cancer InstituteZ. P Pavelic - Roswell Park Cancer InstituteY. M Rustum - Roswell Park Cancer Institute
- Resource Type
- Journal article
- Publication Details
- Cytometry (New York, N.Y.), Vol.5(6), pp.629-635
- DOI
- 10.1002/cyto.990050612
- PMID
- 6518938
- NLM abbreviation
- Cytometry
- ISSN
- 0196-4763
- eISSN
- 1097-0320
- Publisher
- Wiley-Liss
- Language
- English
- Date published
- 1984
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359769102771
Metrics
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