Prolonged treatment with angiotensin 1-7 improves endothelial function in diet-induced obesity

Andreas M Beyer; Deng-Fu Guo; Kamal Rahmouni

doi:10.1097/HJH.0b013e32835ecbe5

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Prolonged treatment with angiotensin 1-7 improves endothelial function in diet-induced obesity

Journal article

Open access

Peer reviewed

Prolonged treatment with angiotensin 1-7 improves endothelial function in diet-induced obesity

Andreas M Beyer, Deng-Fu Guo and Kamal Rahmouni

Journal of hypertension, Vol.31(4), pp.730-738

04/2013

DOI: 10.1097/HJH.0b013e32835ecbe5

PMCID: PMC5684878

PMID: 23425706

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http://doi.org/10.1097/HJH.0b013e32835ecbe5View

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Abstract

The renin-angiotensin system peptides are critically involved in the regulation of endothelial function with important pathological implications. Angiotensin (Ang) 1-7 has many beneficial effects in the vasculature that modulate the cardiovascular risk. Here, we tested the hypothesis that Ang 1-7 has a protective role against the endothelial defects associated with diet-induced obesity (DIO) in mice. Ang 1-7 (with or without Ang II) was delivered subcutaneously for 4 weeks using osmotic minipumps. Vascular studies were performed using aortic rings. Arterial pressure and heart rate were measured in separate cohorts of mice by telemetry. First, we examined whether chronic administration of Ang 1-7 improves the vascular dysfunctions caused by Ang II. Subcutaneous coinfusion of Ang 1-7 significantly attenuates Ang II-induced endothelial dysfunctions. In addition, DIO mice have significant impairment in the endothelium-dependent relaxation. The contractile responses induced by various stimuli, including serotonin and endothelin-1, were differentially altered in DIO mice. Notably, DIO mice treated with Ang 1-7 for 4 weeks displayed significant improvement in the endothelial function as indicated by the increased acetylcholine-induced relaxation. Consistent with this, chronic treatment with Ang 1-7 reversed the increased aortic expression of NAD(P)H oxidase subunits (p22(phox) and p47(phox)) and plasma TBARS associated with DIO mice. In contrast, treatment with Ang 1-7 did not normalize the altered contractions associated with DIO mice. Our data demonstrate a novel role for Ang 1-7 in improving obesity-associated endothelial dysfunction.

Mice, Inbred C57BL

Peptide Fragments - administration & dosage

Angiotensin I - administration & dosage

Endothelium, Vascular - physiopathology

Endothelium, Vascular - drug effects

Male

Obesity - physiopathology

Peptide Fragments - pharmacology

DNA Primers

Reverse Transcriptase Polymerase Chain Reaction

Animals

Diet

Base Sequence

Obesity - etiology

Angiotensin I - pharmacology

Mice

Details

Title: Subtitle: Prolonged treatment with angiotensin 1-7 improves endothelial function in diet-induced obesity
Creators: Andreas M Beyer - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
Deng-Fu Guo
Kamal Rahmouni
Resource Type: Journal article
Publication Details: Journal of hypertension, Vol.31(4), pp.730-738
DOI: 10.1097/HJH.0b013e32835ecbe5
PMID: 23425706
PMCID: PMC5684878
NLM abbreviation: J Hypertens
ISSN: 0263-6352
eISSN: 1473-5598
Publisher: England
Grant note: R01 HL113612 / NHLBI NIH HHS HL014388 / NHLBI NIH HHS T32 HL007121 / NHLBI NIH HHS HL084207 / NHLBI NIH HHS P01 HL014388 / NHLBI NIH HHS HL07121 / NHLBI NIH HHS P01 HL084207 / NHLBI NIH HHS
Language: English
Date published: 04/2013
Academic Unit: Iowa Neuroscience Institute; Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040474602771

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