Promise of Combining Antifungal Agents in Denture Adhesives to Fight Candida Species Infections

Jorge L Garaicoa; Carol L Fischer; Amber M Bates; Julie Holloway; Gustavo Avila-Ortiz; Janet M Guthmiller; Georgia K Johnson; Clark Stanford; Kim A Brogden

doi:10.1111/jopr.12565

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Promise of Combining Antifungal Agents in Denture Adhesives to Fight Candida Species Infections

Journal article

Peer reviewed

Promise of Combining Antifungal Agents in Denture Adhesives to Fight Candida Species Infections

Jorge L Garaicoa, Carol L Fischer, Amber M Bates, Julie Holloway, Gustavo Avila-Ortiz, Janet M Guthmiller, Georgia K Johnson, Clark Stanford and Kim A Brogden

Journal of prosthodontics, Vol.27(8), pp.755-762

10/2018

DOI: 10.1111/jopr.12565

PMCID: PMC5438910

PMID: 27870138

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https://www.ncbi.nlm.nih.gov/pmc/articles/5438910View

Open Access

Abstract

Several complications may arise in patients wearing complete prosthetic appliances, including denture-associated infections and mucosal stomatitis due to Candida species. This study evaluated the activity of anti-Candida agents in denture adhesive and the cytotoxicities of these preparations for primary human gingival epithelial (GE) keratinocytes.\nThe anti-Candida activities of antimicrobial peptides, antimicrobial lipids, and antifungal agents against C. albicans ATCC 64124 or HMV4C were assessed in microdilution assays containing water or 1% denture adhesive. The minimal inhibitory concentrations (MIC) and the minimal bactericidal concentrations (MBC) were determined. The cytotoxicities of denture adhesive compounded with these agents were assessed in 1.0 × 10\nprimary GE keratinocytes in LGM-3 media with resazurin.\nLactoferricin B, SMAP28, sphingosine, dihydrosphingosine, and phytosphingosine in 1% denture adhesive lost antimicrobial activity for C. albicans (p < 0.05). Amphotericin B, chlorhexidine dihydrochloride, chlorhexidine gluconate, fluconazole, and nystatin in 1% denture adhesive or compounded directly into denture adhesive and then diluted to 1% adhesive, did not lose antimicrobial activity. Compounded formulations were not cytotoxic (LD\n> 100.0 μg/ml) against primary human GE keratinocytes.\nAntimicrobial peptides and antimicrobial lipids had diminished activities in 1% adhesive, suggesting that components in adhesives may inactivate local innate immune factors in the oral cavity, possibly predisposing denture wearers to Candida species infections. More importantly, antifungal agents retained their anti-C. albicans activities in denture adhesive, strongly suggesting that antifungal agents could be candidates for inclusion in adhesive formulations and used as prescribed topical treatments for individuals with denture stomatitis.

Denture Retention - methods

Microbial Sensitivity Tests

Antifungal Agents - adverse effects

Candida albicans - drug effects

Adhesives - administration & dosage

Gingiva - drug effects

Humans

Adhesives - therapeutic use

Antifungal Agents - administration & dosage

Candidiasis, Oral - prevention & control

Antifungal Agents - therapeutic use

Details

Title: Subtitle: Promise of Combining Antifungal Agents in Denture Adhesives to Fight Candida Species Infections
Creators: Jorge L Garaicoa - Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University, Portland, OR
Carol L Fischer - Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IA
Amber M Bates - Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IA
Julie Holloway - Department of Prosthodontics, College of Dentistry, The University of Iowa, Iowa City, IA
Gustavo Avila-Ortiz - Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA
Janet M Guthmiller - College of Dentistry, University of Nebraska Medical Center, Lincoln, NE
Georgia K Johnson - Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA
Clark Stanford - College of Dentistry, University of Illinois at Chicago, IL
Kim A Brogden - Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA
Resource Type: Journal article
Publication Details: Journal of prosthodontics, Vol.27(8), pp.755-762
DOI: 10.1111/jopr.12565
PMID: 27870138
PMCID: PMC5438910
NLM abbreviation: J Prosthodont
ISSN: 1059-941X
eISSN: 1532-849X
Publisher: United States
Grant note: NIH\nR01 DE014390 / NIDCR NIH HHS\nT90 DE023520 / NIDCR NIH HHS
Language: English
Date published: 10/2018
Academic Unit: Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Periodontics
Record Identifier: 9984065801302771

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