Journal article
Promoted Bone Healing at a Rabbit Skull Gap Between Autologous Bone Fragment and the Surrounding Intact Bone with Biodegradable Microspheres Containing Transforming Growth Factor-β1
Tissue engineering, Vol.6(4), pp.331-340
08/2000
DOI: 10.1089/107632700418056
Abstract
This study is a trial to promote repairing of the rabbit skull bone gap between an autologous bone flap and the intact bone with biodegradable gelatin microspheres containing transforming growth factor-β1 (TGF-β1). A 10-mm diameter bone defect was prepared in rabbit skulls by drilling out a bone flap of 6 mm in diameter. After a surrounding gap defect of 2 mm was created and treated with 0.5 μg of free TGF-β1 and gelatin microspheres containing 0.5 μg of free TGF-β1, the circular autologous bone flap was placed in the center. Significant bone healing at the gap defect was observed 3 weeks after implantation of the TGF-β1-containing gelatin microspheres. The bone mineral density (BMD) was significantly higher than that of other experimental groups. On the contrary, when applied with free TGF-β1, a fibrous tissue initially infiltrated into the gap defect, resulting in impairing bone healing. The tissue response was similar to that at the defect implanted with empty gelatin microspheres and TGF-β1-free phosphate-buffered saline solution alone. There was more space in the gap-filling bone in the 16-week view than the 3-week view. It is possible that this was an intermediate step along the way toward normal healing and formation of cancellous bone. We conclude that gelatin microspheres containing TGF-β1 show promise as an agent to promote bone regeneration of subcritical size defects between surgically positioned autologous bone flaps and surrounding host bone.
Details
- Title: Subtitle
- Promoted Bone Healing at a Rabbit Skull Gap Between Autologous Bone Fragment and the Surrounding Intact Bone with Biodegradable Microspheres Containing Transforming Growth Factor-β1
- Creators
- Liu Hong - Kyoto UniversityYasuhiko Tabata - Kyoto UniversitySusumu Miyamoto - Kyoto UniversityKeisuke Yamada - Kyoto UniversityIkuo Aoyama - Kyoto UniversityMakoto Tamura - Kaken PharmaceuticalNobuo Hashimoto - Kyoto UniversityYoshito Ikada - Kyoto University
- Resource Type
- Journal article
- Publication Details
- Tissue engineering, Vol.6(4), pp.331-340
- DOI
- 10.1089/107632700418056
- ISSN
- 1076-3279
- eISSN
- 1557-8690
- Language
- English
- Date published
- 08/2000
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367623502771
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