Promotion of a functional B cell germinal center response after Leishmania species co-infection is associated with lesion resolution

Katherine N Gibson-Corley; Paola M Boggiatto; Marie M Bockenstedt; Christine A Petersen; Thomas J Waldschmidt; Douglas E Jones

doi:10.1016/j.ajpath.2012.01.012

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Promotion of a functional B cell germinal center response after Leishmania species co-infection is associated with lesion resolution

Journal article

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Peer reviewed

Promotion of a functional B cell germinal center response after Leishmania species co-infection is associated with lesion resolution

Katherine N Gibson-Corley, Paola M Boggiatto, Marie M Bockenstedt, Christine A Petersen, Thomas J Waldschmidt and Douglas E Jones

The American journal of pathology, Vol.180(5), pp.2009-2017

05/2012

DOI: 10.1016/j.ajpath.2012.01.012

PMCID: PMC3349825

PMID: 22429963

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Abstract

Co-infection of C3HeB/FeJ (C3H) mice with both Leishmania major and Leishmania amazonensis leads to a healed footpad lesion, whereas co-infection of C57BL/6 (B6) mice leads to non-healing lesions. This inability to heal corresponds to a deficiency in B cell stimulation of the macrophage-mediated killing of L. amazonensis in vitro and a less robust antibody response. The mechanism that leads to healing of these lesions is not completely known, although our studies implicate the B cell response as having an important effector function in killing L. amazonensis. To understand more completely this disparate clinical outcome to the same infection, we analyzed the draining lymph node germinal center B cell response between co-infected C3H and B6 mice. There were more germinal center B cells, more antibody isotype-switched germinal center B cells, more memory B cells, and more antigen-specific antibody-producing cells in co-infected C3H mice compared to B6 mice as early as 2 weeks postinfection. Interleukin (IL)-21 production and IL-21 receptor expression in both mouse strains, however, were similar at 2 weeks, suggesting that the difference in the anti-Leishmania response in these mouse strains may be due to differences in T follicular cell commitment or intrinsic B cell differences. These data support the idea that functional B cells are important for healing L. amazonensis in this infectious disease model.

Leishmania major - immunology

Antigens, Protozoan - immunology

Prognosis

Species Specificity

Coinfection - immunology

Germinal Center - immunology

Mice, Inbred C57BL

Immunoglobulin Class Switching - immunology

Receptors, Interleukin-21 - metabolism

Lymph Nodes - immunology

Leishmaniasis, Cutaneous - immunology

Mice, Inbred C3H

Antibodies, Protozoan - biosynthesis

Animals

B-Lymphocytes - immunology

Immunoglobulin G - biosynthesis

Leishmaniasis, Cutaneous - parasitology

Female

Immunologic Memory

Mice

Interleukins - biosynthesis

Leishmania mexicana - immunology

Details

Title: Subtitle: Promotion of a functional B cell germinal center response after Leishmania species co-infection is associated with lesion resolution
Creators: Katherine N Gibson-Corley - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA. katherine-gibson-corley@uiowa.edu
Paola M Boggiatto
Marie M Bockenstedt
Christine A Petersen
Thomas J Waldschmidt
Douglas E Jones
Resource Type: Journal article
Publication Details: The American journal of pathology, Vol.180(5), pp.2009-2017
DOI: 10.1016/j.ajpath.2012.01.012
PMID: 22429963
PMCID: PMC3349825
NLM abbreviation: Am J Pathol
ISSN: 1525-2191
eISSN: 1525-2191
Publisher: United States
Grant note: K08 AI076616 / NIAID NIH HHS
Language: English
Date published: 05/2012
Academic Unit: Epidemiology; Pathology; Internal Medicine; Ophthalmology and Visual Sciences
Record Identifier: 9983996085302771

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