Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins

Mary Beth Harler; Eric Wakshull; Edward J Filardo; Jorge E Albina; Jonathan S Reichner

doi:10.4049/jimmunol.162.11.6792

Back

Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins

Journal article

Peer reviewed

Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins

Mary Beth Harler, Eric Wakshull, Edward J Filardo, Jorge E Albina and Jonathan S Reichner

The Journal of immunology (1950), Vol.162(11), pp.6792-6799

06/01/1999

DOI: 10.4049/jimmunol.162.11.6792

PMID: 10352300

View Online

Abstract

Migration of neutrophils requires sequential adhesive and deadhesive interactions between beta 1 and beta 2 integrins and components of the extracellular matrix. Prompted by reports that describe interaction of soluble beta-glucan with the beta 2 integrin Mac-1, a role for beta-glucan in regulation of integrin-mediated migration was investigated. Neutrophil migration in response to fMLP was assessed using an agarose overlay method with slides precoated with fibronectin (Fn) +/- beta-glucan. On Fn, random migration in excess of directed migration was observed. In contrast, migration on Fn + beta-glucan was directional, with marked diminution of random migration. This conversion of random to directed migration was seen neither when Fn was supplemented with alternative polysaccharides nor when beta-glucan was applied to other components of the extracellular matrix. This effect of beta-glucan was shown to be cation dependent and to be effected by Arg-Gly-Asp-containing peptides consistent with an integrin-mediated event. mAb inhibition studies demonstrate that beta-glucan effects this shift toward directed migration through suppression of migration mediated by Mac-1 and very late Ag 5 and enhancement of very late Ag 3-mediated migration. Adhesion assays suggest that the prochemotactic influence of beta-glucan is due, in part but not entirely, to modulation of PMN adhesion to Fn. In summary, these data support a novel role for beta-glucan in regulation of beta 1- and beta 2-mediated neutrophil migration on Fn.

CD18 Antigens - blood

CD18 Antigens - physiology

Glucans - immunology

CD18 Antigens - immunology

Humans

Cell Migration Inhibition

Integrin beta1 - physiology

Cell Movement - immunology

Chemotaxis, Leukocyte - drug effects

Extracellular Matrix - physiology

Binding, Competitive - immunology

Integrin beta1 - immunology

beta-Glucans

Antibodies, Monoclonal - pharmacology

Neutrophils - drug effects

Neutrophils - immunology

Extracellular Matrix Proteins - physiology

Glucans - pharmacology

Cell Adhesion - immunology

Cell Movement - drug effects

Fibronectins - physiology

Binding Sites, Antibody

Chemotaxis, Leukocyte - immunology

Integrin beta1 - blood

Oligopeptides - pharmacology

Details

Title: Subtitle: Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins
Creators: Mary Beth Harler - Department of Surgery, Division of Surgical Research, Rhode Island Hospital, Brown University School of Medicine, Providence 02903, USA
Eric Wakshull
Edward J Filardo
Jorge E Albina
Jonathan S Reichner
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.162(11), pp.6792-6799
DOI: 10.4049/jimmunol.162.11.6792
PMID: 10352300
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: GM-51493 / NIGMS NIH HHS GM-42859 / NIGMS NIH HHS
Language: English
Date published: 06/01/1999
Academic Unit: Pharmaceutical Sciences and Experimental Therapeutics; Surgery; Internal Medicine
Record Identifier: 9984051871602771

Metrics

21 Record Views

43 Times Cited - Web of Science

See more details