Journal article
Propofol Alleviates DNA Damage Induced by Oxygen Glucose Deprivation and Reperfusion via FoxO1 Nuclear Translocation in H9c2 Cells
Frontiers in physiology, Vol.10, pp.223-223
03/15/2019
DOI: 10.3389/fphys.2019.00223
PMCID: PMC6429026
PMID: 30930790
Abstract
Ischemia/reperfusion (I/R) injury induces irreversible oxidative stress damage to the cardiac myocytes. Many studies have revealed that propofol alleviates the important organelle-mediated injury from oxidative stress
in vitro
. However, it remains unclear whether propofol prevents I/R-induced DNA damage in cardiomyocytes. In our study, we established an oxygen glucose deprivation/reoxygenation (OGD/R) model in H9c2 cells and found that propofol decreased reactive oxygen species (ROS) levels and suppressed cell apoptosis induced by OGD/R in H9c2 cells. In addition, propofol significantly reduced the molecular marker of DNA damage and inhibited double-strand breaks of DNA damage induced by OGD/R in H9c2 cells in a dose-dependent manner. Furthermore, we investigated the molecular mechanisms and demonstrated that propofol inhibited forkhead box O 1 (FoxO1) phosphorylation and increased FoxO1 nuclear translocation through inhibition of protein kinase B (Akt) and adenosine 5’-monophosphate-activated protein kinase (AMPK) pathways. The protective effects of propofol against oxidative stress-induced DNA damage were reversed by silencing FoxO1. Taken together, our results suggest that oxidative stress aggravates DNA damage and apoptosis in H9C2 cells, which can be reversed by propofol
via
FoxO1 nuclear translocation.
Details
- Title: Subtitle
- Propofol Alleviates DNA Damage Induced by Oxygen Glucose Deprivation and Reperfusion via FoxO1 Nuclear Translocation in H9c2 Cells
- Creators
- Dandan Zhou - Shanghai First People's HospitalJinqiang Zhuang - Shanghai Jiao Tong UniversityYihui Wang - Shanghai First People's HospitalDandan Zhao - Shanghai First People's HospitalLidong Zhao - Shanghai First People's HospitalShun Zhu - Shanghai Jiao Tong UniversityJinjun Pu - Shanghai University of Traditional Chinese MedicineMing Yin - Shanghai Jiao Tong UniversityHongyu Zhang - University of BergenZejian Wang - Shanghai Jiao Tong UniversityJiang Hong - , , , , , , , ,
- Resource Type
- Journal article
- Publication Details
- Frontiers in physiology, Vol.10, pp.223-223
- Publisher
- Frontiers Media S.A
- DOI
- 10.3389/fphys.2019.00223
- PMID
- 30930790
- PMCID
- PMC6429026
- ISSN
- 1664-042X
- eISSN
- 1664-042X
- Grant note
- 12XJ3008 / Scientific Research Foundation of Shanghai Jiao Tong University School of Medicine 81570293 / ; 2017sjkjgg31 / Scientific Research Foundation of Shanghai Songjiang District Committee of Science and Technology 2016ZB0205 / Shanghai Health System Key Weak Subject Construction Project 11B24 / Scientific Research Foundation of Shanghai General Hospital
- Language
- English
- Date published
- 03/15/2019
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984696655702771
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