Proposed MIC quality control guidelines for National Committee for Clinical Laboratory Standards susceptibility tests using seven veterinary antimicrobial agents: ceftiofur, enrofloxacin, florfenicol, penicillin G-novobiocin, pirlimycin, premafloxacin, and spectinomycin

Steven A Marshall; R N Jones; Audrey Wanger; John A Washington; Gary V Doern; A L Leber; Thomas H Haugen

doi:10.1128/JCM.34.8.2027-2029.1996

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Proposed MIC quality control guidelines for National Committee for Clinical Laboratory Standards susceptibility tests using seven veterinary antimicrobial agents: ceftiofur, enrofloxacin, florfenicol, penicillin G-novobiocin, pirlimycin, premafloxacin, and spectinomycin

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Proposed MIC quality control guidelines for National Committee for Clinical Laboratory Standards susceptibility tests using seven veterinary antimicrobial agents: ceftiofur, enrofloxacin, florfenicol, penicillin G-novobiocin, pirlimycin, premafloxacin, and spectinomycin

Steven A Marshall, R N Jones, Audrey Wanger, John A Washington, Gary V Doern, A L Leber and Thomas H Haugen

Journal of clinical microbiology, Vol.34(8), pp.2027-2029

08/1996

DOI: 10.1128/JCM.34.8.2027-2029.1996

PMCID: PMC229178

PMID: 8818906

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Abstract

The present multicenter study proposes broth microdilution quality control (QC) ranges for the antimicrobial agents ceftiofur, enrofloxacin, florfenicol, penicillin G-novobiocin, pirlimycin, premafloxacin, and spectinomycin, which are used in veterinary practice. Six separate laboratories tested replicates of National Committee for Clinical Laboratory Standards (NCCLS)-recommended QC organisms (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, and Enterococcus faecalis ATCC 29212) on medium lots both common and unique to all laboratories. The proposed ranges were within 3 or 4 log2 dilution steps of the modal MICs for all organism-antimicrobial agent pairs, depending on their MIC distributions. With > or = 94.7% of all MIC results being within the proposed QC ranges, all combinations tested comply with NCCLS guidelines and all have been accepted by the NCCLS subcommittee developing susceptibility testing procedures for veterinary laboratories.

Details

Title: Subtitle: Proposed MIC quality control guidelines for National Committee for Clinical Laboratory Standards susceptibility tests using seven veterinary antimicrobial agents: ceftiofur, enrofloxacin, florfenicol, penicillin G-novobiocin, pirlimycin, premafloxacin, and spectinomycin
Creators: Steven A Marshall - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA
R N Jones - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA
Audrey Wanger - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA
John A Washington - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA
Gary V Doern - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA
A L Leber - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA
Thomas H Haugen - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.34(8), pp.2027-2029
DOI: 10.1128/JCM.34.8.2027-2029.1996
PMID: 8818906
PMCID: PMC229178
ISSN: 0095-1137
eISSN: 1098-660X
Language: English
Date published: 08/1996
Academic Unit: Epidemiology; Pathology
Record Identifier: 9984047876002771

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