Journal article
Prostaglandin E2 and interleukin-1 concentrations in nicotine-exposed oral keratinocyte cultures
Journal of periodontal research, Vol.32(5), pp.447-454
07/1997
DOI: 10.1111/j.1600-0765.1997.tb00557.x
PMID: 9266496
Abstract
Oral keratinocytes are the first cells in contact with tobacco components and are capable of producing various inflammatory mediators, including PGE2 and IL-1. The purpose of this study was to examine PGE2 and IL-1 concentrations in nicotine-exposed oral keratinocyte cultures. Gingival keratinocyte cultures were established from healthy gingival tissues obtained from 7 subjects. Cultures were divided into 4 groups exposed to serum free medium (control), 0.1 microM, 10 microM or 1 mM nicotine for 4, 24 or 48 h. Using enzyme-linked immunosorbent assays, PGE2 and IL-1 alpha were quantified in culture supernatants; IL-1 alpha and beta were also measured in lysed cells. A repeated measures analysis of variance was used to identify significant differences over time and treatment. Nicotine exposure did not significantly alter PGE2 levels at any given time period; however, PGE2 quantities declined significantly (p = 0.0001) over time. At both 24 and 48 h, IL-1 alpha concentrations in lysates from 1 mM nicotine-exposed cells were significantly (p < 0.01) greater than those for all other treatments. Interleukin-1 alpha quantities also declined significantly (p = 0.037) over time in the cultures. Interleukin-1 beta concentrations were elevated, albeit not significantly, in the 1 mM treated cells at 24 and 48 h. Cell viability, mass and counts were not affected by nicotine treatment; these parameters increased significantly (p < 0.005) over time. In summary, nicotine treatment significantly increased IL-1 alpha concentrations in cultured keratinocytes; however, PGE2 synthesis was not altered. Elevated IL-1 production by keratinocytes may have implications in tobacco-induced lesions, given the central role IL-1 plays in tissue response to injury.
Details
- Title: Subtitle
- Prostaglandin E2 and interleukin-1 concentrations in nicotine-exposed oral keratinocyte cultures
- Creators
- G K Johnson - University of Iowa, College of Dentistry, Iowa City 52242, USAC C Organ
- Resource Type
- Journal article
- Publication Details
- Journal of periodontal research, Vol.32(5), pp.447-454
- Publisher
- United States
- DOI
- 10.1111/j.1600-0765.1997.tb00557.x
- PMID
- 9266496
- ISSN
- 0022-3484
- eISSN
- 1600-0765
- Grant note
- R29DE10153 / NIDCR NIH HHS
- Language
- English
- Date published
- 07/1997
- Academic Unit
- Periodontics
- Record Identifier
- 9984065988902771
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