Journal article
Prostaglandin production in cultured cerebral microvascular smooth muscle is serum dependent
American Journal of Physiology: Cell Physiology, Vol.270(5), pp.C1379-C1387
05/01/1996
DOI: 10.1152/ajpcell.1996.270.5.C1379
PMID: 8967438
Abstract
To expand the understanding of cerebrovascular eicosanoid metabolism, the ability of smooth muscle isolated from murine cerebral microvessels to produce prostaglandins (PGs) was studied in vitro. Cultures from SJL and BALB/c mice produced primarily prostaglandin E2 (PGE2) and I2 (PGI2) in response to exogenous arachidonate and calcium ionophore as well as the agonists acetylcholine and epinephrine. Subconfluent smooth muscle cultures demonstrated a two- to threefold increased capacity to produce PG compared with confluent cultures. In contrast, serum deprivation of smooth muscle caused an 80-90% diminution in both PGE2 and PGI2 production but had no effect on PG release in cerebromicrovascular endothelium. Reintroduction of serum to smooth muscle restored PG production within 6h, and the restoration was inhibited by 1 microM dexamethasone. Message for both prostaglandin H synthase (PGHS)-1 and -2 was detectable in smooth muscle grown in the presence of serum, but PGHS-2 message was not present in serum-deprived cultures. Furthermore, readdition of serum induced a massive increase in PGHS-2 mRNA with only a small increase in PGHS-1 message. The serum induction of PGHS-2 was corroborated by immunohistochemistry and Western blotting. Thus cerebromicrovascular smooth muscle may contribute significantly to the formation of PG under circumstances likely to be present during central nervous system pathologies. The induction of PGHS, particularly PGHS-2, may play a key role in this process.
Details
- Title: Subtitle
- Prostaglandin production in cultured cerebral microvascular smooth muscle is serum dependent
- Creators
- G Rich - Department of Pathology, University of Iowa, Iowa City 52242-1181,USAE. J Yoder - Department of Pathology, University of Iowa, Iowa City 52242-1181,USAL Prokuski - Department of Pathology, University of Iowa, Iowa City 52242-1181,USAS. A Moore - Department of Pathology, University of Iowa, Iowa City 52242-1181,USA
- Resource Type
- Journal article
- Publication Details
- American Journal of Physiology: Cell Physiology, Vol.270(5), pp.C1379-C1387
- DOI
- 10.1152/ajpcell.1996.270.5.C1379
- PMID
- 8967438
- ISSN
- 0363-6143
- eISSN
- 1522-1563
- Language
- English
- Date published
- 05/01/1996
- Academic Unit
- Pathology
- Record Identifier
- 9984046810902771
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