Journal article
Prostaglandins limit nuclear actin to control nucleolar function during oogenesis
Frontiers in cell and developmental biology, Vol.11, 1072456
02/01/2023
DOI: 10.3389/fcell.2023.1072456
PMCID: PMC9981675
PMID: 36875757
Abstract
Prostaglandins (PGs), locally acting lipid signals, regulate female reproduction, including oocyte development. However, the cellular mechanisms of PG action remain largely unknown. One cellular target of PG signaling is the nucleolus. Indeed, across organisms, loss of PGs results in misshapen nucleoli, and changes in nucleolar morphology are indicative of altered nucleolar function. A key role of the nucleolus is to transcribe ribosomal RNA (rRNA) to drive ribosomal biogenesis. Here we take advantage of the robust, in vivo system of Drosophila oogenesis to define the roles and downstream mechanisms whereby PGs regulate the nucleolus. We find that the altered nucleolar morphology due to PG loss is not due to reduced rRNA transcription. Instead, loss of PGs results in increased rRNA transcription and overall protein translation. PGs modulate these nucleolar functions by tightly regulating nuclear actin, which is enriched in the nucleolus. Specifically, we find that loss of PGs results in both increased nucleolar actin and changes in its form. Increasing nuclear actin, by either genetic loss of PG signaling or overexpression of nuclear targeted actin (NLS-actin), results in a round nucleolar morphology. Further, loss of PGs, overexpression of NLS-actin or loss of Exportin 6, all manipulations that increase nuclear actin levels, results in increased RNAPI-dependent transcription. Together these data reveal PGs carefully balance the level and forms of nuclear actin to control the level of nucleolar activity required for producing fertilization competent oocytes.
Details
- Title: Subtitle
- Prostaglandins limit nuclear actin to control nucleolar function during oogenesis
- Creators
- Danielle E. Talbot - Roy J. and Lucille A. Carver College of MedicineBailey J. VormezeeleGarrett C. KimbleDylane M. WinelandDaniel J. KelpschMichelle S. GiedtTina L. Tootle
- Resource Type
- Journal article
- Publication Details
- Frontiers in cell and developmental biology, Vol.11, 1072456
- DOI
- 10.3389/fcell.2023.1072456
- PMID
- 36875757
- PMCID
- PMC9981675
- NLM abbreviation
- Front Cell Dev Biol
- eISSN
- 2296-634X
- Publisher
- Frontiers Media S.A
- Grant note
- DOI: 10.13039/100000076, name: Directorate for Biological Sciences; DOI: 10.13039/100000057, name: National Institute of General Medical Sciences; DOI: 10.13039/100000002, name: National Institutes of Health
- Language
- English
- Date published
- 02/01/2023
- Academic Unit
- Anatomy and Cell Biology; Biology
- Record Identifier
- 9984368061602771
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