Prostaglandins regulate invasive, collective border cell migration

Emily F Fox; Maureen C Lamb; Samuel Q Mellentine; Tina L Tootle

doi:10.1091/mbc.E19-10-0578

Back

Prostaglandins regulate invasive, collective border cell migration

Journal article

Open access

Peer reviewed

Prostaglandins regulate invasive, collective border cell migration

Emily F Fox, Maureen C Lamb, Samuel Q Mellentine and Tina L Tootle

Molecular biology of the cell, Vol.31(15), pp.1584-1594

07/15/2020

DOI: 10.1091/mbc.E19-10-0578

PMCID: PMC7521797

PMID: 32432969

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521797View

Published (Version of record) Open Access

Abstract

While prostaglandins (PGs), short-range lipid signals, regulate single cell migration, their roles in collective migration remain unclear. To address this, we use border cell migration, an invasive, collective migration that occurs during Stage 9 of oogenesis. Pxt is the cyclooxygenase-like enzyme responsible for PG synthesis. Loss of Pxt results in both delayed border cell migration and elongated clusters, whereas somatic Pxt knockdown causes delayed migration and compacted clusters. These findings suggest PGs act in both the border cells and nurse cells, the substrate on which the border cells migrate. As PGs regulate the actin bundler Fascin, and Fascin is required for on-time migration, we assessed whether PGs regulate Fascin to promote border cell migration. Coreduction of Pxt and Fascin results in delayed migration and elongated clusters. The latter may be due to altered cell adhesion, as loss of Pxt or Fascin, or coreduction of both, decreases integrin levels on the border cell membranes. Conversely, integrin localization is unaffected by somatic knockdown of Pxt. Together these data lead to the model that PG signaling controls Fascin in the border cells to promote migration and in the nurse cells to maintain cluster cohesion.

Animals

Carrier Proteins - metabolism

Cell Adhesion

Cell Aggregation

Cell Movement

Drosophila melanogaster - cytology

Drosophila melanogaster - metabolism

Drosophila Proteins - metabolism

Integrins - metabolism

Microfilament Proteins - metabolism

Peroxidases - metabolism

Prostaglandins - metabolism

Signal Transduction

Details

Title: Subtitle: Prostaglandins regulate invasive, collective border cell migration
Creators: Emily F Fox - University of Iowa
Maureen C Lamb - University of Iowa
Samuel Q Mellentine - Roy J. and Lucille A. Carver College of Medicine
Tina L Tootle - University of Iowa
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.31(15), pp.1584-1594
DOI: 10.1091/mbc.E19-10-0578
PMID: 32432969
PMCID: PMC7521797
ISSN: 1059-1524
eISSN: 1939-4586
Grant note: T32 GM008629 / NIGMS NIH HHS P40 OD018537 / NIH HHS R01 GM116885 / NIGMS NIH HHS U41 HG000739 / NHGRI NIH HHS
Language: English
Date published: 07/15/2020
Academic Unit: Anatomy and Cell Biology; Biology
Record Identifier: 9984284452302771

Metrics

18 Record Views

6 Times Cited - Web of Science

See more details