Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture

Christopher M Groen; Asier Jayo; Maddy Parsons; Tina L Tootle

doi:10.1091/mbc.E14-09-1384

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Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture

Journal article

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Peer reviewed

Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture

Christopher M Groen, Asier Jayo, Maddy Parsons and Tina L Tootle

Molecular biology of the cell, Vol.26(10), pp.1901-1917

05/15/2015

DOI: 10.1091/mbc.E14-09-1384

PMCID: PMC4436834

PMID: 25808493

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Abstract

Fascin, a highly conserved actin-bundling protein, localizes and functions at new cellular sites in both Drosophila and multiple mammalian cell types. During Drosophila follicle development, in addition to being cytoplasmic, Fascin is in the nuclei of the germline-derived nurse cells during stages 10B-12 (S10B-12) and at the nuclear periphery during stage 13 (S13). This localization is specific to Fascin, as other actin-binding proteins, Villin and Profilin, do not exhibit the same subcellular distribution. In addition, localization of fascin1 to the nucleus and nuclear periphery is observed in multiple mammalian cell types. Thus the regulation and function of Fascin at these new cellular locations is likely to be highly conserved. In Drosophila, loss of prostaglandin signaling causes a global reduction in nuclear Fascin and a failure to relocalize to the nuclear periphery. Alterations in nuclear Fascin levels result in defects in nucleolar morphology in both Drosophila follicles and cultured mammalian cells, suggesting that nuclear Fascin plays an important role in nucleolar architecture. Given the numerous roles of Fascin in development and disease, including cancer, our novel finding that Fascin has functions within the nucleus sheds new light on the potential roles of Fascin in these contexts.

Signal Transduction

Carrier Proteins - physiology

Drosophila

Humans

Microfilament Proteins - physiology

Ovarian Follicle - ultrastructure

Nuclear Proteins - metabolism

Protein Transport

Prostaglandins - physiology

Animals

Carrier Proteins - metabolism

Cell Nucleolus - ultrastructure

Ovarian Follicle - physiology

Female

Microfilament Proteins - metabolism

Ovarian Follicle - metabolism

Details

Title: Subtitle: Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture
Creators: Christopher M Groen - Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242
Asier Jayo - Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, United Kingdom
Maddy Parsons - Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, United Kingdom
Tina L Tootle - Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242 tina-tootle@uiowa.edu
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.26(10), pp.1901-1917
DOI: 10.1091/mbc.E14-09-1384
PMID: 25808493
PMCID: PMC4436834
NLM abbreviation: Mol Biol Cell
ISSN: 1059-1524
eISSN: 1939-4586
Publisher: American Society for Cell Biology; United States
Grant note: MR/J000647/1 / Medical Research Council UL1 RR024979 / NCRR NIH HHS UL1RR024979 / NCRR NIH HHS
Language: English
Date published: 05/15/2015
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984025307002771

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