Journal article
Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis
Molecular biology of the cell, Vol.25(3), pp.397-411
02/01/2014
DOI: 10.1091/mbc.E13-07-0366
PMCID: PMC3907279
PMID: 24284900
Abstract
Tight regulation of actin remodeling is essential for development, and misregulation results in disease. Cytoskeletal dynamics are regulated by prostaglandins (PGs)—lipid signals. PGs temporally regulate actin remodeling during
Drosophila
oogenesis, at least in part, by modulating the activity of the actin elongation factor Enabled.
Prostaglandins (PGs)—lipid signals produced downstream of cyclooxygenase (COX) enzymes—regulate actin dynamics in cell culture and platelets, but their roles during development are largely unknown. Here we define a new role for Pxt, the
Drosophila
COX-like enzyme, in regulating the actin cytoskeleton—temporal restriction of actin remodeling during oogenesis. PGs are required for actin filament bundle formation during stage 10B (S10B). In addition, loss of Pxt results in extensive early actin remodeling, including actin filaments and aggregates, within the posterior nurse cells of S9 follicles; wild-type follicles exhibit similar structures at a low frequency. Hu li tai shao (Hts-RC) and Villin (Quail), an actin bundler, localize to all early actin structures, whereas Enabled (Ena), an actin elongation factor, preferentially localizes to those in
pxt
mutants. Reduced Ena levels strongly suppress early actin remodeling in
pxt
mutants. Furthermore, loss of Pxt results in reduced Ena localization to the sites of bundle formation during S10B. Together these data lead to a model in which PGs temporally regulate actin remodeling during
Drosophila
oogenesis by controlling Ena localization/activity, such that in S9, PG signaling inhibits, whereas at S10B, it promotes Ena-dependent actin remodeling.
Details
- Title: Subtitle
- Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis
- Creators
- Andrew J Spracklen - Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242Daniel J Kelpsch - Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242Xiang Chen - Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242Cassandra N Spracklen - Department of Epidemiology, College of Public Health, University of Iowa, Iowa City, IA 52242Tina L Tootle - Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242
- Resource Type
- Journal article
- Publication Details
- Molecular biology of the cell, Vol.25(3), pp.397-411
- DOI
- 10.1091/mbc.E13-07-0366
- PMID
- 24284900
- PMCID
- PMC3907279
- NLM abbreviation
- Mol Biol Cell
- ISSN
- 1059-1524
- eISSN
- 1939-4586
- Publisher
- American Society for Cell Biology
- Language
- English
- Date published
- 02/01/2014
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984025342002771
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