Journal article
Protection of K18-hACE2 mice and ferrets against SARS-CoV-2 challenge by a single-dose mucosal immunization with a parainfluenza virus 5–based COVID-19 vaccine
Science advances, Vol.7(27), p.eabi5246
07/2021
DOI: 10.1126/sciadv.abi5246
Abstract
Transmission-blocking vaccines are urgently needed to reduce transmission of SARS-CoV 2, the cause of the COVID-19 pandemic. The upper respiratory tract is an initial site of SARS-CoV-2 infection and, for many individuals, remains the primary site of virus replication. An ideal COVID-19 vaccine should reduce upper respiratory tract virus replication and block transmission as well as protect against severe disease. Here, we optimized a vaccine candidate, parainfluenza virus 5 (PIV5) expressing the SARS-CoV-2 S protein (CVXGA1), and then demonstrated that a single-dose intranasal immunization with CVXGA1 protects against lethal infection of K18-hACE2 mice, a severe disease model. CVXGA1 immunization also prevented virus infection of ferrets and blocked contact transmission. This mucosal vaccine strategy inhibited SARS-CoV-2 replication in the upper respiratory tract, thus preventing disease progression to the lower respiratory tract. A PIV5-based mucosal vaccine provides a strategy to induce protective innate and cellular immune responses and reduce SARS-CoV-2 infection and transmission in populations.
Details
- Title: Subtitle
- Protection of K18-hACE2 mice and ferrets against SARS-CoV-2 challenge by a single-dose mucosal immunization with a parainfluenza virus 5–based COVID-19 vaccine
- Creators
- Dong An - University of GeorgiaKun Li - University of IowaDawne K Rowe - University of GeorgiaMaria Cristina Huertas Diaz - University of GeorgiaEmily F Griffin - University of GeorgiaAshley C Beavis - University of GeorgiaScott K Johnson - University of GeorgiaIan Padykula - University of GeorgiaCheryl A Jones - University of GeorgiaKelsey Briggs - University of GeorgiaGeng Li - University of GeorgiaYuan Lin - University of GeorgiaJiachen Huang - University of GeorgiaJarrod Mousa - University of GeorgiaMelinda Brindley - University of GeorgiaKaori Sakamoto - University of GeorgiaDavid K Meyerholz - University of IowaPaul B McCray Jr - University of IowaS. Mark Tompkins - University of GeorgiaBiao He - University of Georgia
- Resource Type
- Journal article
- Publication Details
- Science advances, Vol.7(27), p.eabi5246
- DOI
- 10.1126/sciadv.abi5246
- ISSN
- 2375-2548
- eISSN
- 2375-2548
- Grant note
- name: CyanVac LLC
- Language
- English
- Date published
- 07/2021
- Academic Unit
- Pathology; Microbiology and Immunology; Internal Medicine; Stead Family Department of Pediatrics; Pulmonary Medicine
- Record Identifier
- 9984186532802771
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