Journal article
Protective CD8 T cell-mediated immunity against influenza A virus infection following influenza virus-like particle vaccination
The Journal of immunology (1950), Vol.191(5), pp.2486-2494
09/01/2013
DOI: 10.4049/jimmunol.1300954
PMCID: PMC3777857
PMID: 23885108
Abstract
The development of influenza A virus (IAV) vaccines capable of inducing cytotoxic CD8 T cell responses could potentially provide superior, long-term protection against multiple, heterologous strains of IAV. Although prior studies demonstrated the effectiveness of baculovirus-derived virus-like particle (VLP) vaccination in generating Ab-mediated protection, the role that CD8 T cell immunity plays in overall VLP-mediated protection is less-well understood. In this article, we demonstrate that intranasal vaccination of mice with a VLP containing the hemagglutinin and matrix 1 proteins of IAV/PR/8/34 leads to a significant increase in hemagglutinin 533-specific CD8 T cells in the lungs and protection following subsequent homologous challenge with IAV. VLP-mediated protection was significantly reduced by CD8 T cell depletion, indicating a critical role for CD8 T cells in protective immunity. Importantly, our results show that VLP vaccine-induced CD8 T cell-mediated protection is not limited to homologous IAV strains. VLP vaccination leads to an increase in protection following heterosubtypic challenge with a strain of IAV that avoids vaccine-induced neutralizing Abs but contains conserved, immunodominant CD8 T cell epitopes. Overall, our results demonstrate the ability of influenza protein-containing VLPs to prime IAV-specific CD8 T cell responses that contribute to protection from homo- and heterosubtypic IAV infections. These results further suggest that vaccination strategies focused on the development of cross-protective CD8 T cell responses may contribute to the development of "universal" IAV vaccines.
Details
- Title: Subtitle
- Protective CD8 T cell-mediated immunity against influenza A virus infection following influenza virus-like particle vaccination
- Creators
- Emily A Hemann - Department of Pathology and Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242, USASang-Moo KangKevin L Legge
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.191(5), pp.2486-2494
- DOI
- 10.4049/jimmunol.1300954
- PMID
- 23885108
- PMCID
- PMC3777857
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- United States
- Grant note
- R01 AI071085 / NIAID NIH HHS R21 AA021205 / NIAAA NIH HHS T32 AI007485 / NIAID NIH HHS R01 AI093772 / NIAID NIH HHS AI071085 / NIAID NIH HHS R56 AI071085 / NIAID NIH HHS T32 AI007533 / NIAID NIH HHS
- Language
- English
- Date published
- 09/01/2013
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047745502771
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