Protective effect of melatonin against mitomycin C-induced genotoxic damage in peripheral blood of rats

S Ortega-Gutiérrez; M López-Vicente; F Lostalé; L Fuentes-Broto; E Martínez-Ballarín; J J García

doi:10.1155/2009/791432

Back

Protective effect of melatonin against mitomycin C-induced genotoxic damage in peripheral blood of rats

Journal article

Open access

Protective effect of melatonin against mitomycin C-induced genotoxic damage in peripheral blood of rats

S Ortega-Gutiérrez, M López-Vicente, F Lostalé, L Fuentes-Broto, E Martínez-Ballarín and J J García

Journal of biomedicine & biotechnology, Vol.2009, 791432

2009

DOI: 10.1155/2009/791432

PMCID: PMC2764378

PMID: 19859567

Files and links (1)

url

https://doi.org/10.1155/2009/791432View

Published (Version of record) Open Access

Abstract

Mitomycin C (MMC) generates free radicals when metabolized. We investigated the effect of melatonin against MMC-induced genotoxicity in polychromatic erythrocytes and MMC-induced lipid peroxidation in brain and liver homogenates. Rats (N = 36) were classified into 4 groups: control, melatonin, MMC, and MMC + melatonin. Melatonin and MMC doses of 10 mg/kg and 2 mg/kg, respectively, were injected intraperitoneally. Peripheral blood samples were collected at 0, 24, 48, 72, and 96 hours posttreatment and homogenates were obtained at 96 hours posttreatment. The number of micronucleated polychromatic erythrocytes (MN-PCE) per 1000 PCE was used as a genotoxic marker. Malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA) levels were used as an index of lipid peroxidation. The MMC group showed a significant increase in MN-PCE at 24, 48, 72, and 96 hours that was significantly reduced with melatonin begin coadministrated. No significant differences were found in lipid peroxidation. Our results indicate that MMC-induced genotoxicity can be reduced by melatonin.

Malondialdehyde - metabolism

Mutagens - toxicity

Liver - metabolism

Rats

Rats, Sprague-Dawley

Erythrocytes - drug effects

Brain - drug effects

Brain - metabolism

Micronucleus Tests

Animals

Liver - drug effects

Erythrocytes - metabolism

Lipid Peroxidation - drug effects

Mitomycin - antagonists & inhibitors

Mitomycin - toxicity

In Vitro Techniques

Melatonin - pharmacology

Details

Title: Subtitle: Protective effect of melatonin against mitomycin C-induced genotoxic damage in peripheral blood of rats
Creators: S Ortega-Gutiérrez - Department of Pharmacology and Physiology, University of Zaragoza, 50009 Zaragoza, Spain
M López-Vicente
F Lostalé
L Fuentes-Broto
E Martínez-Ballarín
J J García
Resource Type: Journal article
Publication Details: Journal of biomedicine & biotechnology, Vol.2009, 791432
DOI: 10.1155/2009/791432
PMID: 19859567
PMCID: PMC2764378
NLM abbreviation: J Biomed Biotechnol
ISSN: 1110-7243
eISSN: 1110-7251
Publisher: United States
Grant note: DOI: 10.13039/501100004587, name: Instituto de Salud Carlos III, award: RD06/0013/1017, B40
Language: English
Date published: 2009
Academic Unit: Neurology; Radiology; Iowa Neuroscience Institute; Neurosurgery
Record Identifier: 9984020606702771

Metrics

21 Record Views

22 Times Cited - Web of Science