Journal article
Protective effects of enalapril in streptozotocin-induced diabetic rat: studies of DNA damage, apoptosis and expression of CCN2 in the heart, kidney and liver
Journal of applied toxicology, Vol.32(9), pp.662-672
09/01/2012
DOI: 10.1002/jat.1670
PMID: 21416479
Abstract
Diabetes mellitus is characterized by hyperglycemia, which induces oxidative stress and perturbs a number of pathways, leading to tissue injury. One of the pathological responses to tissue injury is the development of fibrosis and cell death. Enalapril is a non-thiol angiotensin-converting enzyme inhibitor that is commonly used in the treatment of diabetes-associated hypertension. The present study examines the possible beneficial effects of enalapril on the development of diabetes associated fibrosis and DNA damage in rats. SpragueDawley rats (250?+/-?10?g) were used in the study. Enalapril (10?mg kg-1 per oral) was administered for four consecutive weeks to the streptozotocin (STZ)-induced diabetic rats. After 4?weeks, all the animals were sacrificed and comet assay (normal and modified) was performed to detect the normal as well as oxidative DNA damage. Expression of profibrotic marker CCN2 and fibrosis was examined in the heart, kidney and liver of diabetic rats. Enalapril treatment significantly restored the malondialdehyde and glutathione content as well as the DNA damage in the heart, kidney and liver of diabetic rat. Significant decrease in the expression of CCN2 was observed in the heart, kidney and liver of diabetic rat receiving enalapril treatment as compared with the diabetic group. Further, the enalapril treatment led to significant decrease in the fibrosis and CCN2 expression in the diabetic group as compared with control. The results of the present study clearly demonstrate that enalapril ameliorates the DNA damage, cell death and expression of CCN2 in the heart, kidney and liver of the STZ-induced diabetic rat. Copyright (c) 2011 John Wiley & Sons, Ltd.
Details
- Title: Subtitle
- Protective effects of enalapril in streptozotocin-induced diabetic rat: studies of DNA damage, apoptosis and expression of CCN2 in the heart, kidney and liver
- Creators
- S. Kushwaha - National Institute of Pharmaceutical Education and ResearchA. Vikram - National Institute of Pharmaceutical Education and Research Department of Pharmacology and Toxicology Punjab IndiaG. B. Jena - National Institute of Pharmaceutical Education and Research Department of Pharmacology and Toxicology Punjab India
- Resource Type
- Journal article
- Publication Details
- Journal of applied toxicology, Vol.32(9), pp.662-672
- Publisher
- Wiley
- DOI
- 10.1002/jat.1670
- PMID
- 21416479
- ISSN
- 0260-437X
- eISSN
- 1099-1263
- Number of pages
- 11
- Grant note
- National Institute of Pharmaceutical Education and Research (NIPER), S.A.S. Nagar, Mohali, Punjab, India SR/SO/HS-83/2008 / Department of Science and Technology (DST), India; Department of Science & Technology (India)
- Language
- English
- Date published
- 09/01/2012
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984359694102771
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