Protective function and durability of mouse lymph node-resident memory CD8+ T cells

Scott M Anthony; Natalija Van Braeckel-Budimir; Steven J Moioffer; Stephanie van de Wall; Qiang Shan; Rahul Vijay; Ramakrishna Sompallae; Stacey M Hartwig; Isaac J Jensen; Steven M Varga; Noah S Butler; Hai-Hui Xue; Vladimir P Badovinac; John T Harty

doi:10.7554/eLife.68662

Back

Protective function and durability of mouse lymph node-resident memory CD8+ T cells

Journal article

Open access

Peer reviewed

Protective function and durability of mouse lymph node-resident memory CD8+ T cells

Scott M Anthony, Natalija Van Braeckel-Budimir, Steven J Moioffer, Stephanie van de Wall, Qiang Shan, Rahul Vijay, Ramakrishna Sompallae, Stacey M Hartwig, Isaac J Jensen, Steven M Varga, …

eLife, Vol.10, e68662

06/18/2021

DOI: 10.7554/eLife.68662

PMID: 34143731

Files and links (1)

url

https://doi.org/10.7554/eLife.68662View

Published (Version of record) Open Access

Abstract

Protective lung tissue-resident memory CD8+T cells (Trm) form after influenza A virus (IAV) infection. We show that IAV infection of mice generates CD69+CD103+and other memory CD8+T cell populations in lung-draining mediastinal lymph nodes (mLNs) from circulating naive or memory CD8+T cells. Repeated antigen exposure, mimicking seasonal IAV infections, generates quaternary memory (4M) CD8+T cells that protect mLN from viral infection better than 1M CD8+T cells. Better protection by 4M CD8+T cells associates with enhanced granzyme A/B expression and stable maintenance of mLN CD69+CD103+4M CD8+T cells, vs the steady decline of CD69+CD103+1M CD8+T cells, paralleling the durability of protective CD69+CD103+4M vs 1M in the lung after IAV infection. Coordinated upregulation in canonical Trm-associated genes occurs in circulating 4M vs 1M populations without the enrichment of canonical downregulated Trm genes. Thus, repeated antigen exposure arms circulating memory CD8+T cells with enhanced capacity to form long-lived populations of Trm that enhance control of viral infections of the mLN.

Details

Title: Subtitle: Protective function and durability of mouse lymph node-resident memory CD8+ T cells
Creators: Scott M Anthony - University of Iowa
Natalija Van Braeckel-Budimir - University of Iowa
Steven J Moioffer - University of Iowa
Stephanie van de Wall - University of Iowa
Qiang Shan - University of Iowa
Rahul Vijay - University of Iowa
Ramakrishna Sompallae - University of Iowa
Stacey M Hartwig - University of Iowa
Isaac J Jensen - University of Iowa
Steven M Varga - University of Iowa
Noah S Butler - University of Iowa
Hai-Hui Xue - University of Iowa
Vladimir P Badovinac - University of Iowa
John T Harty - University of Iowa
Resource Type: Journal article
Publication Details: eLife, Vol.10, e68662
DOI: 10.7554/eLife.68662
PMID: 34143731
NLM abbreviation: Elife
ISSN: 2050-084X
eISSN: 2050-084X
Grant note: DOI: 10.13039/100000052, name: NIH Office of the Director, award: AI42767; DOI: 10.13039/100000052, name: NIH Office of the Director, award: AI11453; DOI: 10.13039/100000052, name: NIH Office of the Director, award: AI25446; DOI: 10.13039/100000052, name: NIH Office of the Director, award: AI27481; DOI: 10.13039/100000052, name: NIH Office of the Director, award: AI124093; DOI: 10.13039/100000052, name: NIH Office of the Director, award: GM134880; DOI: 10.13039/100000052, name: NIH Office of the Director, award: AI121080; DOI: 10.13039/100000052, name: NIH Office of the Director, award: AI129874; DOI: 10.13039/100000052, name: NIH Office of the Director, award: T32HL007; DOI: 10.13039/100000052, name: NIH Office of the Director, award: T32AI1005511; DOI: 10.13039/100016416, name: Veterans Affairs Council, R.O.C., award: BX002903
Language: English
Date published: 06/18/2021
Academic Unit: Molecular Physiology and Biophysics; Graduate College Admin and Gen; Microbiology and Immunology; Pathology
Record Identifier: 9984180920102771

Metrics

31 Record Views

22 Times Cited - Web of Science