Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection, and Spatiotemporal Dynamic Studies of Protein-Protein Interactions in Living Cells

Stephen W Michnick; Christian R Landry; Emmanuel D Levy; Guillaume Diss; Po Hien Ear; Jacqueline Kowarzyk; Mohan K Malleshaiah; Vincent Messier; Emmanuelle Tchekanda

doi:10.1101/pdb.top083543

Journal article

Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection, and Spatiotemporal Dynamic Studies of Protein-Protein Interactions in Living Cells

Stephen W Michnick, Christian R Landry, Emmanuel D Levy, Guillaume Diss, Po Hien Ear, Jacqueline Kowarzyk, Mohan K Malleshaiah, Vincent Messier and Emmanuelle Tchekanda

Cold Spring Harbor protocols, Vol.2016(11), pp.917-919

11/01/2016

DOI: 10.1101/pdb.top083543

PMID: 27803260

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Abstract

Protein-fragment complementation assays (PCAs) comprise a family of assays that can be used to study protein-protein interactions (PPIs), conformation changes, and protein complex dimensions. We developed PCAs to provide simple and direct methods for the study of PPIs in any living cell, subcellular compartments or membranes, multicellular organisms, or in vitro. Because they are complete assays, requiring no cell-specific components other than reporter fragments, they can be applied in any context. PCAs provide a general strategy for the detection of proteins expressed at endogenous levels within appropriate subcellular compartments and with normal posttranslational modifications, in virtually any cell type or organism under any conditions. Here we introduce a number of applications of PCAs in budding yeast, Saccharomyces cerevisiae These applications represent the full range of PPI characteristics that might be studied, from simple detection on a large scale to visualization of spatiotemporal dynamics.

Protein Interaction Mapping - methods

Saccharomyces cerevisiae - chemistry

Saccharomyces cerevisiae Proteins - chemistry

Saccharomyces cerevisiae Proteins - metabolism

Spatio-Temporal Analysis

Details

Title: Subtitle: Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection, and Spatiotemporal Dynamic Studies of Protein-Protein Interactions in Living Cells
Creators: Stephen W Michnick - Université de Montréal
Christian R Landry - Université Laval
Emmanuel D Levy - Weizmann Institute of Science
Guillaume Diss - Université Laval
Po Hien Ear - Université de Montréal
Jacqueline Kowarzyk - Université de Montréal
Mohan K Malleshaiah - Université de Montréal
Vincent Messier - Université de Montréal
Emmanuelle Tchekanda - Université de Montréal
Resource Type: Journal article
Publication Details: Cold Spring Harbor protocols, Vol.2016(11), pp.917-919
DOI: 10.1101/pdb.top083543
PMID: 27803260
ISSN: 1940-3402
eISSN: 1559-6095
Language: English
Date published: 11/01/2016
Academic Unit: Surgery
Record Identifier: 9984322818702771

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