Journal article
Protein Identification Using Sorcerer 2 and SEQUEST
Current Protocols in Bioinformatics, Vol.28(1), pp.13.3.1-13.3.21
12/2009
DOI: 10.1002/0471250953.bi1303s28
PMID: 19957274
Abstract
Sage‐N's Sorcerer 2 provides an integrated data analysis system for comprehensive protein identification and characterization. It runs on a proprietary version of SEQUESTR, the most widely used search engine for identifying proteins in complex mixtures. The protocol presented here describes the basic steps performed to process mass spectrometric data with Sorcerer 2 and how to analyze results using TPP and Scaffold. The unit also provides an overview of the SEQUESTR algorithm, along with Sorcerer‐SEQUESTR enhancements, and a discussion of data filtering methods, important considerations in data interpretation, and additional resources that can be of assistance to users running Sorcerer and interpreting SEQUESTR results. Curr. Protoc. Bioinform. 28:13.3.1‐13.3.21. © 2009 by John Wiley & Sons, Inc.
Details
- Title: Subtitle
- Protein Identification Using Sorcerer 2 and SEQUEST
- Creators
- Deborah H. Lundgren - Department of Cell Biology, Center for Vascular Biology, University of Connecticut Health CenterHarryl Martinez - Department of Molecular Physiology and Biophysics, University of Iowa Carver College of MedicineMichael E. Wright - University of Iowa, Molecular Physiology and BiophysicsDavid K. Han - Department of Cell Biology, Center for Vascular Biology, University of Connecticut Health Center
- Resource Type
- Journal article
- Publication Details
- Current Protocols in Bioinformatics, Vol.28(1), pp.13.3.1-13.3.21
- DOI
- 10.1002/0471250953.bi1303s28
- PMID
- 19957274
- ISSN
- 1934-3396
- eISSN
- 1934-340X
- Number of pages
- 21
- Language
- English
- Date published
- 12/2009
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984297492002771
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