Journal article
Protein Zero Is Necessary for E-Cadherin-Mediated Adherens Junction Formation in Schwann Cells
Molecular and cellular neurosciences, Vol.18(6), pp.606-618
12/2001
DOI: 10.1006/mcne.2001.1041
PMID: 11749037
Abstract
Protein Zero (P0), the major structural protein in the peripheral nervous system (PNS) myelin, acts as a homotypic adhesion molecule and is thought to mediate compaction of adjacent wraps of myelin membrane. E-Cadherin, a calcium-dependent adhesion molecule, is also expressed in myelinating Schwann cells in the PNS and is involved in forming adherens junctions between adjacent loops of membrane at the paranode. To determine the relationship, if any, between P0-mediated and cadherin-mediated adhesion during myelination, we investigated the expression of E-cadherin and its binding partner, β-catenin, in sciatic nerve of mice lacking P0 (P0−/−). We find that in P0−/− peripheral myelin neither E-cadherin nor β-catenin are localized to paranodes, but are instead found in small puncta throughout the Schwann cell. In addition, only occasional, often rudimentary, adherens junctions are formed. Analysis of E-cadherin and β-catenin expression during nerve development demonstrates that E-cadherin and β-catenin are localized to the paranodal region after the onset of myelin compaction. Interestingly, axoglial junction formation is normal in P0−/− nerve. Taken together, these data demonstrate that P0 is necessary for the formation of adherens junctions but not axoglial junctions in myelinating Schwann cells.
Details
- Title: Subtitle
- Protein Zero Is Necessary for E-Cadherin-Mediated Adherens Junction Formation in Schwann Cells
- Creators
- Daniela Maria Menichella - Institute of Neurology, IRCCS Ospedale Maggiore, Centro Dino Ferrari, University of Milan, Milan, ItalyEdgardo J Arroyo - Department of Neurology, University of Pennsylvania, Philadelphia, PennsylvaniaRajeshwar Awatramani - Graduate Program in Cell and Molecular Biology, University of Pennsylvania, School of Medicine, Philadelphia, PennsylvaniaTheodore Xu - Department of Neurology, University of Pennsylvania, Philadelphia, PennsylvaniaPierluigi Baron - Institute of Neurology, IRCCS Ospedale Maggiore, Centro Dino Ferrari, University of Milan, Milan, ItalyJean-Michael Vallat - Department of Neurology, University Hospital, Limoges, FranceJanne Balsamo - Department of Biological Sciences, University of Iowa, IowaJack Lilien - Department of Biological Sciences, University of Iowa, IowaGuglielmo Scarlato - Institute of Neurology, IRCCS Ospedale Maggiore, Centro Dino Ferrari, University of Milan, Milan, ItalyJohn Kamholz - Department of Neurology and Center of Medicine and Genetics, Wayne State University, Detroit, MichiganSteven S Scherer - Department of Neurology, University of Pennsylvania, Philadelphia, PennsylvaniaMichael E Shy - Department of Neurology and Center of Medicine and Genetics, Wayne State University, Detroit, Michigan
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular neurosciences, Vol.18(6), pp.606-618
- Publisher
- Elsevier Inc
- DOI
- 10.1006/mcne.2001.1041
- PMID
- 11749037
- ISSN
- 1044-7431
- eISSN
- 1095-9327
- Language
- English
- Date published
- 12/2001
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Psychiatry; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984020758102771
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