Journal article
Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs
American journal of physiology. Lung cellular and molecular physiology, Vol.305(3), pp.L256-L266
08/01/2013
DOI: 10.1152/ajplung.00056.2013
PMCID: PMC3743012
PMID: 23709621
Abstract
The airway mucosa and the alveolar surface form dynamic interfaces between the lung and the external environment. The epithelial cells lining these barriers elaborate a thin liquid layer containing secreted peptides and proteins that contribute to host defense and other functions. The goal of this study was to develop and apply methods to define the proteome of porcine lung lining liquid, in part, by leveraging the wealth of information in the
Sus scrofa
database of Ensembl gene, transcript, and protein model predictions. We developed an optimized workflow for detection of secreted proteins in porcine bronchoalveolar lavage (BAL) fluid and in methacholine-induced tracheal secretions [airway surface liquid (ASL)]. We detected 674 and 3,858 unique porcine-specific proteins in BAL and ASL, respectively. This proteome was composed of proteins representing a diverse range of molecular classes and biological processes, including host defense, molecular transport, cell communication, cytoskeletal, and metabolic functions. Specifically, we detected a significant number of secreted proteins with known or predicted roles in innate and adaptive immunity, microbial killing, or other aspects of host defense. In greatly expanding the known proteome of the lung lining fluid in the pig, this study provides a valuable resource for future studies using this important animal model of pulmonary physiology and disease.
Details
- Title: Subtitle
- Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs
- Creators
- Jennifer A Bartlett - Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IowaMatthew E Albertolle - Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California; andChristine Wohlford-Lenane - Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IowaAlejandro A Pezzulo - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IowaJoseph Zabner - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IowaRichard K Niles - Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California; andSusan J Fisher - Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California; andPaul B McCray - Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IowaKatherine E Williams - Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California; and
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.305(3), pp.L256-L266
- DOI
- 10.1152/ajplung.00056.2013
- PMID
- 23709621
- PMCID
- PMC3743012
- NLM abbreviation
- Am J Physiol Lung Cell Mol Physiol
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Publisher
- American Physiological Society
- Language
- English
- Date published
- 08/01/2013
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Internal Medicine
- Record Identifier
- 9984093364702771
Metrics
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