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Protein kinase C-ζ mediates lung injury induced by diesel exhaust particles
Journal article   Open access   Peer reviewed

Protein kinase C-ζ mediates lung injury induced by diesel exhaust particles

Juan C Caraballo, Jennifer Borcherding, Peter S Thorne and Alejandro P Comellas
American journal of respiratory cell and molecular biology, Vol.48(3), pp.306-313
03/2013
DOI: 10.1165/rcmb.2012-0056OC
PMCID: PMC3604088
PMID: 23221045
url
https://doi.org/10.1165/rcmb.2012-0056OCView
Published (Version of record) Open Access

Abstract

Recently, we reported that diesel exhaust particles (DEPs) disrupt tight junctions (TJs) in alveolar epithelial cells (AECs) via an increase in reactive oxygen species (ROS). In this study, we investigated the role of protein kinase C (PKC)-ζ activation in DEP-induced lung injury. C57/bl6 mice were instilled intratracheally with 50 μl of saline containing 100 μg of DEPs or titanium dioxide (TiO2). Twenty-four hours later, bronchoalveolar lavage was performed to assess neutrophil counts and protein concentrations. In addition, in vitro experiments were performed in primary rat and human AECs exposed to DEPs (50 μg/cm(2)) for 3 hours. Transepithelial electrical conductance was measured, and TJ protein association was analyzed by immunoprecipitation. To determine whether the overexpression of antioxidants prevented DEP-induced lung injury, AECs and mice were infected with adenoviruses containing catalase and manganese superoxide dismutase (MnSOD) plasmids. In vivo, the overexpression of catalase and MnSOD prevented DEP-induced neutrophil recruitment. The inhibition of PKC-ζ activation also prevented DEP-induced neutrophil recruitment in vivo. In vitro, DEPs activated PKC-ζ in AECs, but not in alveolar macrophages. Using a specific myristolated PKC-ζ pseudosubstrate pepetide (PKC-ζ ps), we showed that PKC-ζ mediated the DEP-induced dissociation of occludin and zonula occludin-1 (ZO1) in rat and human AECs. In addition, the overexpression of constitutively active PKC-ζ induced the dissociation of occludin and ZO1 in AECs. DEP-induced TJ disruption occurs via PKC-ζ. TJ disruption seems to be in part responsible for DEP-induced lung injury.
 Vehicle Emissions - toxicity Epithelial Cells - metabolism Epithelial Cells - drug effects Humans Bronchoalveolar Lavage Male Occludin - metabolism Lung Injury - enzymology Protein Kinase C - metabolism Lung Injury - metabolism Zonula Occludens-1 Protein - metabolism Neutrophils - metabolism Superoxide Dismutase - metabolism Mice, Inbred C57BL Neutrophils - drug effects Cells, Cultured Rats Protein Kinase C - antagonists & inhibitors Rats, Sprague-Dawley Catalase - metabolism Titanium - toxicity Animals Macrophages, Alveolar - drug effects Mice Macrophages, Alveolar - metabolism Lung Injury - chemically induced

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