Logo image
IRO Home Research units Researcher Profiles
Sign in
Protein mixed-disulfides in cardiac cells. S-thiolation of soluble proteins in response to diamide
Journal article   Peer reviewed

Protein mixed-disulfides in cardiac cells. S-thiolation of soluble proteins in response to diamide

Linda M Grimm, Mark W Collison, Rory A Fisher and James A Thomas
Biochimica et biophysica acta. Molecular cell research, Vol.844(1), pp.50-54
01/18/1985
DOI: 10.1016/0167-4889(85)90232-0
PMID: 3967051

View Online

Abstract

Protein mixed-disulfides in cultured rat heart cells were analyzed by gel electrophoresis under conditions that eliminated artifactual formation of these protein derivatives. Protein S-thiolation (protein mixed-disulfide formation) was detectable under normal culture conditions. Diamide oxidized intracellular glutathione in these cells and produced extensive protein S-thiolation. The specificity of this protein modification indicates a role in the regulation of cardiac metabolism.
Muscle Proteins Animals Molecular Weight Oxidation-Reduction Glutathione - metabolism Cystine - metabolism Myocardium - metabolism Disulfides Rats Diamide - pharmacology Azo Compounds - pharmacology

Details

Metrics

Logo image