Journal article
Proteolysis of a Negative Regulator of Innate Immunity Is Dependent on Resistance Genes in Tomato and Nicotiana benthamiana and Induced by Multiple Bacterial Effectors[W][OA]
The Plant cell, Vol.21(8), pp.2458-2472
08/01/2009
DOI: 10.1105/tpc.107.056044
PMCID: PMC2751963
PMID: 19671880
Abstract
RPM1-interacting protein 4 (RIN4), a negative regulator of the basal defense response in plants, is targeted by multiple bacterial virulence effectors. We show that RIN4 degradation is induced by the effector AvrPto from
Pseudomonas syringae
and that this degradation in Solanaceous plants is dependent on the resistance protein, Pto, a protein kinase, and Prf, a nucleotide binding site–leucine-rich repeat protein. Our data demonstrate overlap between two of the best-characterized pathways for recognition of pathogen virulence effectors in plants. RIN4 interacts with multiple plant signaling components and bacterial effectors in yeast and in planta
.
AvrPto induces an endogenous proteolytic activity in both tomato (
Solanum lycopersicum
) and
Nicotiana benthamiana
that degrades RIN4 and requires the consensus site cleaved by the protease effector AvrRpt2. The interaction between AvrPto and Pto, but not the kinase activity of Pto, is required for proteolysis of RIN4. Analysis of many of the effectors comprising the secretome of
P. syringae
pv
tomato
DC3000 led to the identification of two additional sequence-unrelated effectors that can also induce degradation of RIN4. Therefore, multiple bacterial effectors besides AvrRpt2 elicit proteolysis of RIN4 in planta.
Details
- Title: Subtitle
- Proteolysis of a Negative Regulator of Innate Immunity Is Dependent on Resistance Genes in Tomato and Nicotiana benthamiana and Induced by Multiple Bacterial Effectors[W][OA]
- Creators
- Yao Luo - University of California, DavisKatherine S. Caldwell - University of California, DavisTadeusz Wroblewski - University of California, DavisMichael E. Wright - University of California, DavisRichard W. Michelmore - University of California, Davis
- Resource Type
- Journal article
- Publication Details
- The Plant cell, Vol.21(8), pp.2458-2472
- DOI
- 10.1105/tpc.107.056044
- PMID
- 19671880
- PMCID
- PMC2751963
- NLM abbreviation
- Plant Cell
- ISSN
- 1040-4651
- eISSN
- 1532-298X
- Publisher
- American Society of Plant Biologists
- Language
- English
- Date published
- 08/01/2009
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984297600902771
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