Proteome sequencing goes deep

Alicia L Richards; Anna E Merrill; Joshua J Coon

doi:10.1016/j.cbpa.2014.10.017

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Journal article

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Peer reviewed

Proteome sequencing goes deep

Alicia L Richards, Anna E Merrill and Joshua J Coon

Current opinion in chemical biology, Vol.24, pp.11-17

02/2015

DOI: 10.1016/j.cbpa.2014.10.017

PMCID: PMC4308434

PMID: 25461719

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https://www.ncbi.nlm.nih.gov/pmc/articles/4308434View

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Abstract

Advances in mass spectrometry (MS) have transformed the scope and impact of protein characterization efforts. Identifying hundreds of proteins from rather simple biological matrices, such as yeast, was a daunting task just a few decades ago. Now, expression of more than half of the estimated ∼20,000 human protein coding genes can be confirmed in record time and from minute sample quantities. Access to proteomic information at such unprecedented depths has been fueled by strides in every stage of the shotgun proteomics workflow-from sample processing to data analysis-and promises to revolutionize our understanding of the causes and consequences of proteome variation.

Proteome - genetics

Mass Spectrometry - instrumentation

Humans

Proteomics - instrumentation

Peptides - genetics

Proteome - analysis

Proteomics - methods

Mass Spectrometry - methods

Peptides - analysis

Details

Title: Subtitle: Proteome sequencing goes deep
Creators: Alicia L Richards - Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI 53706, United States
Anna E Merrill - Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI 53706, United States
Joshua J Coon - Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI 53706, United States; Department of Biomolecular Chemistry, University of Wisconsin-Madison, 420 Henry Mall, Madison, WI 53706, United States. Electronic address: jcoon@chem.wisc.edu
Resource Type: Journal article
Publication Details: Current opinion in chemical biology, Vol.24, pp.11-17
DOI: 10.1016/j.cbpa.2014.10.017
PMID: 25461719
PMCID: PMC4308434
NLM abbreviation: Curr Opin Chem Biol
ISSN: 1367-5931
eISSN: 1879-0402
Publisher: England
Grant note: T15LM007359 / NLM NIH HHS R01 GM080148 / NIGMS NIH HHS 5T32HG002760 / NHGRI NIH HHS T32 HG002760 / NHGRI NIH HHS T15 LM007359 / NLM NIH HHS
Language: English
Date published: 02/2015
Academic Unit: Pathology; Injury Prevention Research Center
Record Identifier: 9984046800502771

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