Journal article
Proteomic Analysis of Ethene-Enriched Groundwater Microcosms from a Vinyl Chloride-Contaminated Site
Environmental science & technology, Vol.44(5), pp.1594-1601
03/01/2010
DOI: 10.1021/es903033r
PMID: 20121086
Abstract
Contamination of groundwater with vinyl chloride (VC), a known human carcinogen, is a common environmental problem at plastics manufacturing, dry cleaning, and military sites. At many sites, there is the potential to cleanup VC groundwater plumes with aerobic VC-oxidizing microorganisms (e.g., methanotrophs, etheneotrophs, and VC-assimilating bacteria). Environmental biotechnologies that reveal the presence and activity of VC-oxidizing bacteria in contaminated groundwater samples would provide valuable lines of evidence that bioremediation of VC is occurring at a site. We applied targeted shotgun mass spectrometry-based proteomic methods to ethene-enriched groundwater microcosms from a VC-contaminated site. Polypeptides from the enzymes alkene monooxygenase (EtnC) and epoxyalkane:CoM transferase (EtnE), both of which are expressed by aerobic etheneotrophs and VC-assimilating bacteria, were identified in 7 of the 14 samples analyzed. Bioinformatic analysis revealed that 2 EtnC and 5 EtnE peptides were unique to deduced EtnC and EtnE sequences from two different cultivated strains. In addition, several partial EtnE genes sequenced from microcosms matched with observed EtnE peptides. Our results have revealed broader etheneotroph functional gene diversity and demonstrate the feasibility, speed, and accuracy of applying a targeted metaproteomics approach to identifying protein biomarkers from etheneotrophs in complex environmental samples.
Details
- Title: Subtitle
- Proteomic Analysis of Ethene-Enriched Groundwater Microcosms from a Vinyl Chloride-Contaminated Site
- Creators
- Adina S ChuangYang Oh JinLaura S SchmidtYalan LiSamuel FogelDonna SmolerTimothy E Mattes
- Resource Type
- Journal article
- Publication Details
- Environmental science & technology, Vol.44(5), pp.1594-1601
- DOI
- 10.1021/es903033r
- PMID
- 20121086
- NLM abbreviation
- Environ Sci Technol
- ISSN
- 0013-936X
- eISSN
- 1520-5851
- Publisher
- American Chemical Society
- Language
- English
- Date published
- 03/01/2010
- Academic Unit
- Civil and Environmental Engineering
- Record Identifier
- 9983991983702771
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