Proteomic Approaches to Identify and Characterize Alterations to the Mitochondrial Proteome in Alcoholic Liver Disease

Shannon M. Bailey; Kelly K. Andringa; Aimee Landar; Victor M. Darley-Usmar

doi:10.1007/978-1-59745-242-7_24

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Journal article

Proteomic Approaches to Identify and Characterize Alterations to the Mitochondrial Proteome in Alcoholic Liver Disease

Shannon M. Bailey, Kelly K. Andringa, Aimee Landar and Victor M. Darley-Usmar

Methods in molecular biology (Clifton, N.J.), Vol.447, pp.369-380

2008

DOI: 10.1007/978-1-59745-242-7_24

PMCID: PMC2935618

PMID: 18369930

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https://www.ncbi.nlm.nih.gov/pmc/articles/2935618View

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Abstract

Mitochondrial dysfunction is recognized as a contributing factor to a number of diseases including chronic alcohol induced hepatotoxicity. While there is a detailed understanding of the metabolic pathways and proteins of the liver mitochondrion, little is known of how changes in the mitochondrial proteome contribute to the development of hepatic pathologies. In this short overview the insights gained from study of changes in the mitochondrial proteome in alcoholic liver disease will be described. Profiling the liver mitochondrial proteome has the potential to shed light on the alcohol-mediated molecular defects responsible for mitochondrial and cellular dysfunction. The methods presented herein demonstrate the power of using complementary proteomics approaches, i.e. 2-D IEF/SDS-PAGE and BN-PAGE, to identify changes in the abundance of mitochondrial proteins following chronic alcohol consumption. This proteomic data can then be integrated into a logical and mechanistic framework to further our understanding of the role of mitochondrial dysfunction in the pathogenesis of alcohol induced liver disease.

Details

Title: Subtitle: Proteomic Approaches to Identify and Characterize Alterations to the Mitochondrial Proteome in Alcoholic Liver Disease
Creators: Shannon M. Bailey - University of Alabama at Birmingham
Kelly K. Andringa - University of Alabama at Birmingham
Aimee Landar - University of Alabama at Birmingham
Victor M. Darley-Usmar - University of Alabama at Birmingham
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.447, pp.369-380
DOI: 10.1007/978-1-59745-242-7_24
PMID: 18369930
PMCID: PMC2935618
NLM abbreviation: Methods Mol Biol
eISBN: 1597452424; 9781597452427
ISSN: 1064-3745
eISSN: 1940-6029
Language: English
Date published: 2008
Academic Unit: Orthopedics and Rehabilitation
Record Identifier: 9984548858502771

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