Journal article
Proteomic Interactions in the Mouse Vitreous-Retina Complex
PloS one, Vol.8(11), pp.e82140-e82140
11/29/2013
DOI: 10.1371/journal.pone.0082140
PMCID: PMC3843729
PMID: 24312404
Abstract
Purpose: Human vitreoretinal diseases are due to presumed abnormal mechanical interactions between the vitreous and retina, and translational models are limited. This study determined whether nonstructural proteins and potential retinal biomarkers were expressed by the normal mouse vitreous and retina.
Methods: Vitreous and retina samples from mice were collected by evisceration and analyzed by liquid chromatography-tandem mass spectrometry. Identified proteins were further analyzed for differential expression and functional interactions using bioinformatic software.
Results: We identified 1,680 unique proteins in the retina and 675 unique proteins in the vitreous. Unbiased clustering identified protein pathways that distinguish retina from vitreous including oxidative phosphorylation and neurofilament cytoskeletal remodeling, whereas the vitreous expressed oxidative stress and innate immunology pathways. Some intracellular protein pathways were found in both retina and vitreous, such as glycolysis and gluconeogenesis and neuronal signaling, suggesting proteins might be shuttled between the retina and vitreous. We also identified human disease biomarkers represented in the mouse vitreous and retina, including carbonic anhydrase-2 and 3, crystallins, macrophage inhibitory factor, glutathione peroxidase, peroxiredoxins, S100 precursors, and von Willebrand factor.
Conclusions: Our analysis suggests the vitreous expresses nonstructural proteins that functionally interact with the retina to manage oxidative stress, immune reactions, and intracellular proteins may be exchanged between the retina and vitreous. This novel proteomic dataset can be used for investigating human vitreoretinopathies in mouse models. Validation of vitreoretinal biomarkers for human ocular diseases will provide a critical tool for diagnostics and an avenue for therapeutics.
Details
- Title: Subtitle
- Proteomic Interactions in the Mouse Vitreous-Retina Complex
- Creators
- Jessica M. Skeie - University of IowaVinit B. Mahajan - University of Iowa
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.8(11), pp.e82140-e82140
- Publisher
- Public Library Science
- DOI
- 10.1371/journal.pone.0082140
- PMID
- 24312404
- PMCID
- PMC3843729
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Number of pages
- 14
- Grant note
- F32EY022280 / NATIONAL EYE INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Eye Institute (NEI) 1F32EY022280-01A1; K08EY020530 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 11/29/2013
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9984627233602771
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