Journal article
Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism
The Journal of immunology (1950), Vol.175(4), pp.2237-2243
08/15/2005
DOI: 10.4049/jimmunol.175.4.2237
PMID: 16081791
Abstract
Vesicle transport is a fundamental mechanism of communication in the CNS. In this study we characterized a novel type of vesicle released by murine brain microglial cells: microglial exosomes. Analysis of their protein content identified several enzymes, chaperones, tetraspanins, and membrane receptors previously reported in B cells and dendritic cell-derived exosomes. Additionally, microglia-derived exosomes expressed the aminopeptidase CD13 and the lactate transporter MCT-1. Exosomal CD13 was metabolically active in cleaving leucine- and methionine-enkephalins peptides by releasing the N-terminal tyrosine. Cleaved neuropeptides were unable to bind to the neuronal opioid receptor as assessed by cAMP response. Microglial exosomal vesicles may represent an important, previously unrecognized, cellular communication system in an organ in which cell motility is highly restricted.
Details
- Title: Subtitle
- Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism
- Creators
- Ilaria Potolicchio - Department of Pathology, Albert Einstein College of Medicine, New York, NY 10461, USAGregory J CarvenXiaonan XuChristopher StippRichiard J RieseLawrence J SternLaura Santambrogio
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.175(4), pp.2237-2243
- DOI
- 10.4049/jimmunol.175.4.2237
- PMID
- 16081791
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- R01AI048832 / NIAID NIH HHS K08AI01555 / NIAID NIH HHS CA86712 / NCI NIH HHS R01AI38996 / NIAID NIH HHS
- Language
- English
- Date published
- 08/15/2005
- Academic Unit
- Molecular Physiology and Biophysics; Biology
- Record Identifier
- 9984217419202771
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