Journal article
Proteomic examination of Leishmania chagasi plasma membrane proteins: contrast between avirulent and virulent (metacyclic) parasite forms
Proteomics. Clinical applications, Vol.4(1), pp.4-16
01/2010
DOI: 10.1002/prca.200900050
PMCID: PMC3049999
PMID: 21137013
Abstract
Leishmaniasis annually leads to two million new cases with 59 thousand deaths worldwide. Promastigotes of the causative
Leishmania
spp. develop from procyclic (log) to the highly virulent metacyclic stage within the sand fly vector. We hypothesized that proteins important for promastigote virulence might be uniquely represented in the plasma membrane of metacyclic, but not log, promastigotes. Purified metacyclic promastigotes from stationary phase cultures of
Leishmania chagasi
were applied to prepare membrane preparations either by surface biotinylation-streptavidin affinity separation or by octyl glucoside detergent extraction. These membrane fractions were enriched over 130 and 250 fold, respectively, estimated by western blotting for the plasma membrane marker MSP. A total of 447 or 33 proteins were identified by surface biotinylation or detergent extraction, respectively, by LC-MS/MS. Confocal microscopy suggested the difference between the lists was due to the fact that proteins localized both on the surface membrane and within the flagellar pocket were accessible to surface biotinylation. Using detergent extraction, we found different proteins were present in membrane proteins of logarithmic stage compared to metacyclic stage promastigotes. Several dozens were stage specific. These data provide a foundation for identifying virulence factors in the plasma membranes of
Leishmania
spp. promastigotes during metacyclogenesis.
Details
- Title: Subtitle
- Proteomic examination of Leishmania chagasi plasma membrane proteins: contrast between avirulent and virulent (metacyclic) parasite forms
- Creators
- Chaoqun Yao - Department of Veterinary Sciences and Wyoming State Veterinary Laboratory, University of Wyoming, Laramie, WY 82070Yalan Li - Department of Veterinary Sciences and Wyoming State Veterinary Laboratory, University of Wyoming, Laramie, WY 82070John E Donelson - Department of Veterinary Sciences and Wyoming State Veterinary Laboratory, University of Wyoming, Laramie, WY 82070Mary E Wilson - Department of Veterinary Sciences and Wyoming State Veterinary Laboratory, University of Wyoming, Laramie, WY 82070
- Resource Type
- Journal article
- Publication Details
- Proteomics. Clinical applications, Vol.4(1), pp.4-16
- DOI
- 10.1002/prca.200900050
- PMID
- 21137013
- PMCID
- PMC3049999
- NLM abbreviation
- Proteomics Clin Appl
- ISSN
- 1862-8346
- eISSN
- 1862-8354
- Grant note
- name: National Institutes of Health, two Merit Review grants (C. Y. and M. E. W.), an MERP, award: AI32135, AI059451, AI45540, AI048822; DOI: 10.13039/100000738, name: Department of Veterans' Affairs
- Language
- English
- Date published
- 01/2010
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984001210702771
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