Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease

B D Jones; H L Mobley

doi:10.1128/jb.171.12.6414-6422.1989

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Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease

Journal article

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Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease

B D Jones and H L Mobley

Journal of bacteriology, Vol.171(12), pp.6414-6422

12/1989

DOI: 10.1128/jb.171.12.6414-6422.1989

PMCID: PMC210529

PMID: 2687233

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Abstract

Proteus mirabilis, a common cause of urinary tract infection, produces a potent urease that hydrolyzes urea to NH3 and CO2, initiating kidney stone formation. Urease genes, which were localized to a 7.6-kilobase-pair region of DNA, were sequenced by using the dideoxy method. Six open reading frames were found within a region of 4,952 base pairs which were predicted to encode polypeptides of 31.0 (ureD), 11.0 (ureA), 12.2 (ureB), 61.0 (ureC), 17.9 (ureE), and 23.0 (ureF) kilodaltons (kDa). Each open reading frame was preceded by a ribosome-binding site, with the exception of ureE. Putative promoterlike sequences were identified upstream of ureD, ureA, and ureF. Possible termination sites were found downstream of ureD, ureC, and ureF. Structural subunits of the enzyme were encoded by ureA, ureB, and ureC and were translated from a single transcript in the order of 11.0, 12.2, and 61.0 kDa. When the deduced amino acid sequences of the P. mirabilis urease subunits were compared with the amino acid sequence of the jack bean urease, significant amino acid similarity was observed (58% exact matches; 73% exact plus conservative replacements). The 11.0-kDa polypeptide aligned with the N-terminal residues of the plant enzyme, the 12.2-kDa polypeptide lined up with internal residues, and the 61.0-kDa polypeptide matched with the C-terminal residues, suggesting an evolutionary relationship of the urease genes of jack bean and P. mirabilis.

Amino Acid Sequence

Genes, Bacterial

Proteus mirabilis - genetics

Genes

Molecular Sequence Data

Restriction Mapping

Sequence Homology, Nucleic Acid

Plants - enzymology

Proteus mirabilis - enzymology

Plants - genetics

Escherichia coli - genetics

Plasmids

Base Sequence

Cloning, Molecular

Protein Conformation

Urease - genetics

Details

Title: Subtitle: Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease
Creators: B D Jones - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201
H L Mobley
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.171(12), pp.6414-6422
DOI: 10.1128/jb.171.12.6414-6422.1989
PMID: 2687233
PMCID: PMC210529
NLM abbreviation: J Bacteriol
ISSN: 0021-9193
eISSN: 1098-5530
Grant note: AG04393 / NIA NIH HHS AI23328 / NIAID NIH HHS
Language: English
Date published: 12/1989
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083200802771

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