Journal article
Protist Homologs of the Meiotic Spo11 Gene and Topoisomerase VI reveal an Evolutionary History of Gene Duplication and Lineage-Specific Loss
Molecular biology and evolution, Vol.24(12), pp.2827-2841
12/2007
DOI: 10.1093/molbev/msm217
PMID: 17921483
Abstract
Spo11 is a meiotic protein of fundamental importance as it is a conserved meiosis-specific transesterase required for meiotic recombination initiation in fungi, animals, and plants. Spo11 is homologous to the archaebacterial topoisomerase VIA (Top6A) gene, and its homologs are broadly distributed among eukaryotes, with some eukaryotes having more than one homolog. However, the evolutionary relationships among these genes are unclear, with some debate as to whether eukaryotic homologs originated by lateral gene transfer. We have identified and characterized protist Spo11 homologs by degenerate polymerase chain reaction (PCR) and sequencing and by analyses of sequences from public databases. Our phylogenetic analyses show that Spo11 homologs evolved by two ancient eukaryotic gene duplication events prior to the last common ancestor of extant eukaryotes, resulting in three eukaryotic paralogs: Spo11-1, Spo11-2, and Spo11-3. Spo11-1 orthologs encode meiosis-specific proteins and are distributed broadly among eukaryotic lineages, though Spo11-1 is absent from some protists. This absence coincides with the presence of Spo11-2 orthologs, which are meiosis-specific in Arabidopsis and are found in plants, red algae, and some protists but absent in animals and fungi. Spo11-3 encodes a Top6A subunit that interacts with topoisomerase VIB (Top6B) subunits, which together play a role in vegetative growth in Arabidopsis. We identified Spo11-3 (Top6A) and Top6B homologs in plants, red algae, and a few protists, establishing a broader distribution of these genes among eukaryotes, indicating their likely vertical descent followed by lineage-specific loss.
Details
- Title: Subtitle
- Protist Homologs of the Meiotic Spo11 Gene and Topoisomerase VI reveal an Evolutionary History of Gene Duplication and Lineage-Specific Loss
- Creators
- Shehre-Banoo Malik - Department of Biological Sciences, Roy J. Carver Center for Comparative Genomics, University of IowaMarilee A Ramesh - †Department of Biology, Roanoke CollegeAlissa M Hulstrand - Department of Biological Sciences, Roy J. Carver Center for Comparative Genomics, University of IowaJohn M Logsdon - Department of Biological Sciences, Roy J. Carver Center for Comparative Genomics, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular biology and evolution, Vol.24(12), pp.2827-2841
- Publisher
- Oxford University Press
- DOI
- 10.1093/molbev/msm217
- PMID
- 17921483
- ISSN
- 0737-4038
- eISSN
- 1537-1719
- Language
- English
- Date published
- 12/2007
- Academic Unit
- Biology
- Record Identifier
- 9984217416902771
Metrics
15 Record Views