Journal article
Proto-Oncogene mRNA Levels and Activities of Multiple Transcription Factors in C3H 10T 1/2 Murine Embryonic Fibroblasts Exposed to 835.62 and 847.74 MHz Cellular Phone Communication Frequency Radiation
Radiation research, Vol.151(3), pp.300-309
03/1999
DOI: 10.2307/3579942
PMID: 10073668
Abstract
This study was designed to determine whether two differently modulated radiofrequencies of the type generally used in cellular phone communications could elicit a general stress response in a biological system. The two modulations and frequencies studied were a frequency-modulated continuous wave (FMCW) with a carrier frequency of 835.62 MHz and a code division multiple-access (CDMA) modulation centered on 847.74 MHz. Changes in proto-oncogene expression, determined by measuring Fos, Jun, and Myc mRNA levels as well as by the DNA-binding activity of the AP1, AP2 and NF-κB transcription factors, were used as indicators of a general stress response. The effect of radiofrequency exposure on proto-oncogene expression was assessed (1) in exponentially growing C3H 10T 1/2 mouse embryo fibroblasts during their transition to plateau phase and (2) during transition of serum-deprived cells to the proliferation cycle after serum stimulation. Exposure of serum-deprived cells to 835.62 MHz FMCW or 847.74 MHz CDMA microwaves (at an average specific absorption rate, SAR, of 0.6 W/kg) did not significantly change the kinetics of proto-oncogene expression after serum stimulation. Similarly, these exposures did not affect either the Jun and Myc mRNA levels or the DNA-binding activity of AP1, AP2 and NF-κB in exponential cells during transit to plateau-phase growth. Therefore, these results suggest that the radiofrequency exposure is unlikely to elicit a general stress response in cells of this cell line under these conditions. However, statistically significant increases (∼2-fold, P = 0.001) in Fos mRNA levels were detected in exponential cells in transit to the plateau phase and in plateau-phase cells exposed to 835.62 MHz FMCW microwaves. For 847.74 MHz CDMA exposure, the increase was 1.4-fold (P = 0.04). This increase in Fos expression suggests that expression of specific genes could be affected by radiofrequency exposure.
Details
- Title: Subtitle
- Proto-Oncogene mRNA Levels and Activities of Multiple Transcription Factors in C3H 10T 1/2 Murine Embryonic Fibroblasts Exposed to 835.62 and 847.74 MHz Cellular Phone Communication Frequency Radiation
- Creators
- Prabhat C GoswamiLee D AlbeeAzemat J ParsianJack D BatyEduardo G MorosWilliam F PickardJoseph L Roti RotiClayton R Hunt
- Resource Type
- Journal article
- Publication Details
- Radiation research, Vol.151(3), pp.300-309
- DOI
- 10.2307/3579942
- PMID
- 10073668
- ISSN
- 0033-7587
- eISSN
- 1938-5404
- Language
- English
- Date published
- 03/1999
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047852802771
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