Journal article
Protocol Protocol for inducing monomicrobial sepsis in mice with uropathogenic E. coli
STAR protocols, Vol.5(3), 103206
09/20/2024
DOI: 10.1016/j.xpro.2024.103206
PMCID: PMC11338187
PMID: 39068653
Abstract
Bacterial infections are the primary cause of pathogenic sepsis. An uropathogenic E. coli (UPEC) model of monomicrobial sepsis represents a useful tool for interrogating the host immune response to this pathogen. Here, we present a protocol for inducing monomicrobial sepsis in mice using UPEC. We describe steps for preparing the bacteria, delivering UPEC into mice, and monitoring the mice post-infection. We then detail procedures for measuring cytokine response
Details
- Title: Subtitle
- Protocol Protocol for inducing monomicrobial sepsis in mice with uropathogenic E. coli
- Creators
- Caleb Y. Kim - University of MinnesotaShravan Kumar Kannan - University of IowaVladimir P. Badovinac - University of IowaThomas S. Griffith - University of Minnesota
- Resource Type
- Journal article
- Publication Details
- STAR protocols, Vol.5(3), 103206
- DOI
- 10.1016/j.xpro.2024.103206
- PMID
- 39068653
- PMCID
- PMC11338187
- NLM abbreviation
- STAR Protoc
- ISSN
- 2666-1667
- eISSN
- 2666-1667
- Publisher
- Elsevier
- Number of pages
- 14
- Grant note
- GM140881; GM134880 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA IK6BX006192 / Research Career Scientist award from the Department of Veterans Affairs; US Department of Veterans Affairs BX001324 / Veterans Administration Merit Review Award; US Department of Veterans Affairs
- Language
- English
- Date published
- 09/20/2024
- Academic Unit
- Pathology
- Record Identifier
- 9984697760302771
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