Journal article
Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
STAR protocols, Vol.1(2), pp.100068-100068
09/18/2020
DOI: 10.1016/j.xpro.2020.100068
PMCID: PMC7580194
PMID: 33111106
Abstract
Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to study lung diseases characterized by PNEC dysfunction.
For complete details on the use and execution of this protocol, please refer to Hor et al. (2020).
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•Efficient generation of iPNECs from human iPSCs•Air-liquid-interface culture augments iPNEC specification•Continuous Notch inhibition increases iPNEC specification•iPNEC gene expression signature is highly concordant with primary human fetal PNECs
Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to study lung diseases characterized by PNEC dysfunction.
Details
- Title: Subtitle
- Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
- Creators
- Pooja Hor - Hastings CenterJustin K. Ichida - University of Southern CaliforniaZea Borok - University of Southern CaliforniaAmy L. Ryan - Hastings Center
- Resource Type
- Journal article
- Publication Details
- STAR protocols, Vol.1(2), pp.100068-100068
- DOI
- 10.1016/j.xpro.2020.100068
- PMID
- 33111106
- PMCID
- PMC7580194
- NLM abbreviation
- STAR Protoc
- ISSN
- 2666-1667
- eISSN
- 2666-1667
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100000002, name: NIH, award: R35HL135747, R00NS077435, R01NS097850; DOI: 10.13039/100003194, name: New York Stem Cell Foundation; name: USC Broad Innovation Award; DOI: 10.13039/100015603, name: Hastings Foundation
- Language
- English
- Date published
- 09/18/2020
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284330902771
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